Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4013089..4013686 Replicon chromosome
Accession NZ_CP086423
Organism Klebsiella pneumoniae strain AE-2247421-T20-MAC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LNN44_RS19715 Protein ID WP_004142563.1
Coordinates 4013369..4013686 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LNN44_RS19710 Protein ID WP_004142561.1
Coordinates 4013089..4013376 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN44_RS19680 (4009170) 4009170..4009418 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LNN44_RS19685 (4009436) 4009436..4009777 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LNN44_RS19690 (4009808) 4009808..4010923 - 1116 Protein_3876 MBL fold metallo-hydrolase -
LNN44_RS19695 (4011102) 4011102..4011683 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
LNN44_RS19700 (4011683) 4011683..4012051 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LNN44_RS19705 (4012171) 4012171..4012824 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
LNN44_RS19710 (4013089) 4013089..4013376 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LNN44_RS19715 (4013369) 4013369..4013686 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LNN44_RS19720 (4013871) 4013871..4014914 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
LNN44_RS19725 (4015581) 4015581..4016447 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
LNN44_RS19730 (4016556) 4016556..4017983 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T223285 WP_004142563.1 NZ_CP086423:c4013686-4013369 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T223285 NZ_CP118630:588270-588641 [Pantoea dispersa]
GTGTGGCAGGTTGAAGCAACAGATCGGTTTTGGAAATGGTTGCAGGCTCAGGATGAAGCGTTGCGTCTCGACGTGCTTGC
CGCCCTGAAGCTTCTGGCGCAGGACGGTCCGCATCTCGGTCGCCCTTTCGTTGATACTCTGATGTTATCGCGGGTGCCCA
ATATGAAAGAGCTACGCGTGCAAAGTAAAGGTCGTCCCATCCGCAGCTTTTTTGTTTTCGATCCGCGCCGCCATGCGATT
GTGCTGTGTGCCGGCAACAAACAGGGTAAAAATCAGAAGCGTTTCTATCAGCAGATGCTTCACATTGCCGAAACCGAATA
CCATCATCATCTGAACGCAATCGGGGAAACTCATAATGAAAACCTTGGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT223285 WP_004142561.1 NZ_CP086423:c4013376-4013089 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT223285 NZ_CP118630:588625-588924 [Pantoea dispersa]
ATGAAAACCTTGGATGAGATGCTGGCGGCGCTGACGCCCGAACAGCAACATCAGGTCGATGAACGCGTCAGCCAGATGCG
TCTGGAATACCAGCTTTATCAATTACGTGAGCAGATGCAGCGGACTCAGCGTCAGCAAGCGCAGGCCATGCACATTGCCC
AGTCATCTGTGGCGGCGATGGAAGCGCGTGGTGCAGAGTTAAAAATTTCCACGCTGAAGCGCTACATTGAGGCGCTGGGT
GGAGAGCTGACGCTGGGTGTAAAGATGCCGGGTGGCCAGCATGTTGACTTAATTATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References