Detailed information of TA system TA022323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 40887..41477
• Replicon: plasmid pOANT03
• Accession: NC_009671
• Organism: Ochrobactrum anthropi ATCC 49188

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A6X893
• Locus tag: OANT_RS24560
• Protein ID: WP_011983054.1
• Coordinates: 40887..41168 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OANT_RS24565
• Protein ID: WP_011983055.1
• Coordinates: 41181..41477 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OANT_RS24540	36499..37542	+	1044	WP_011983049.1	plasmid partitioning protein RepB	-
OANT_RS24545	37697..38920	+	1224	WP_011983050.1	replication initiation protein RepC	-
OANT_RS24550	39022..39447	+	426	WP_011983051.1	DUF5086 domain-containing protein	-
OANT_RS24555	39462..39842	+	381	WP_011983052.1	DUF2195 family protein	-
OANT_RS26110	40096..40725	-	630	WP_011983053.1	SH3 domain-containing protein	-
OANT_RS24560	40887..41168	+	282	WP_011983054.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OANT_RS24565	41181..41477	+	297	WP_011983055.1	HigA family addiction module antidote protein	Antitoxin
OANT_RS24570	41856..42368	+	513	WP_011983057.1	hypothetical protein	-
OANT_RS24580	43740..44378	+	639	WP_011983059.1	hypothetical protein	-
OANT_RS24585	44473..44790	+	318	WP_040128186.1	hypothetical protein	-
OANT_RS24590	44806..45821	-	1016	Protein_42	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93589	93589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10479.00 Da
• Isoelectric Point: 9.0323

>T22323 WP_011983054.1 NC_009671:40887-41168 [Ochrobactrum anthropi ATCC 49188]
MIRSYKDARTEAVAAGKAPKGFPADLIRTTVRKLTMIDSAVILDDLRSPPGNRLEALKGDRKGQHSIRINDQWRICFVWT
DAGAEQIEIVDYH

Nucleotide

• Length: 282 bp

>T22323 NC_009671:40887-41168 [Ochrobactrum anthropi ATCC 49188]
ATGATCCGTTCTTACAAAGATGCCAGAACCGAAGCCGTTGCAGCCGGCAAAGCGCCAAAGGGGTTCCCGGCAGATTTGAT
AAGAACAACTGTTCGCAAACTTACCATGATCGATAGTGCTGTAATTCTCGATGACCTTCGCTCTCCTCCCGGAAACAGGC
TGGAAGCTCTGAAGGGGGACCGTAAAGGGCAGCACTCGATTCGCATCAACGATCAGTGGCGCATCTGTTTCGTTTGGACC
GATGCTGGCGCTGAACAAATCGAAATTGTTGACTATCACTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10929.70 Da
• Isoelectric Point: 7.2026

>AT22323 WP_011983055.1 NC_009671:41181-41477 [Ochrobactrum anthropi ATCC 49188]
MTSKLPPVHPGEILREEYLIPLGMSAGALAKNLNVPRTRIERLATEQTAVTPDTALRLAKFFRTTPEFWMNLQSGYDLKT
EAVAIQDQLLAIHELKAA

Nucleotide

• Length: 297 bp

>AT22323 NC_009671:41181-41477 [Ochrobactrum anthropi ATCC 49188]
ATGACCAGTAAACTCCCCCCGGTCCATCCCGGCGAAATCCTTCGGGAAGAATATCTCATCCCGCTCGGGATGAGTGCAGG
TGCGCTTGCCAAGAATCTGAATGTTCCTCGCACTCGAATTGAGCGTCTGGCGACAGAACAGACTGCCGTTACACCTGATA
CGGCTCTGCGCCTTGCGAAGTTCTTCCGCACCACGCCCGAATTCTGGATGAATTTGCAGTCAGGTTACGACCTTAAAACA
GAGGCGGTAGCTATTCAGGATCAGCTTTTAGCCATCCATGAACTGAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A6X893