Detailed information of TA system TA223228

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2733406..2733665
• Replicon: chromosome
• Accession: NZ_CP086411
• Organism: Enterococcus faecalis strain R5

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: -
• Locus tag: LMJ46_RS13655
• Protein ID: WP_075551663.1
• Coordinates: 2733564..2733665 (-)
• Length: 34 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2733406..2733615 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ46_RS13625 (2728659)	2728659..2729612	-	954	WP_002370087.1	siderophore ABC transporter substrate-binding protein	-
LMJ46_RS13630 (2729651)	2729651..2730406	-	756	WP_002354962.1	ATP-binding cassette domain-containing protein	-
LMJ46_RS13635 (2730403)	2730403..2731368	-	966	WP_002371665.1	iron chelate uptake ABC transporter family permease subunit	-
LMJ46_RS13640 (2731365)	2731365..2732312	-	948	WP_002354960.1	iron chelate uptake ABC transporter family permease subunit	-
LMJ46_RS13645 (2732497)	2732497..2732949	+	453	WP_002389410.1	YueI family protein	-
- (2732975)	2732975..2733182	+	208	NuclAT_2	-	-
- (2733012)	2733012..2733186	+	175	NuclAT_6	-	-
LMJ46_RS13650 (2733131)	2733131..2733232	-	102	WP_021164441.1	putative holin-like toxin	-
- (2733406)	2733406..2733615	+	210	NuclAT_3	-	Antitoxin
- (2733440)	2733440..2733619	+	180	NuclAT_5	-	-
LMJ46_RS13655 (2733564)	2733564..2733665	-	102	WP_075551663.1	putative holin-like toxin	Toxin
LMJ46_RS13660 (2733855)	2733855..2736125	-	2271	WP_002389492.1	bifunctional glutamate--cysteine ligase/glutathione synthetase	-
LMJ46_RS13665 (2736296)	2736296..2736796	+	501	WP_002372831.1	cysteine hydrolase family protein	-
LMJ46_RS13670 (2737195)	2737195..2738091	+	897	WP_002389477.1	YitT family protein	-
LMJ46_RS13675 (2738142)	2738142..2738540	-	399	WP_002354951.1	glyoxalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-33)

Sequences

Toxin

Protein

• Length: 34 a.a.
• Molecular weight: 3599.34 Da
• Isoelectric Point: 4.5869

>T223228 WP_075551663.1 NZ_CP086411:c2733665-2733564 [Enterococcus faecalis]
MSIEAALELMISFAAFVALLIFGILEATKNDKK

Nucleotide

• Length: 102 bp

>T223228 NZ_CP118601:288673-288948 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTTCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 210 bp

>AT223228 NZ_CP086411:2733406-2733615 [Enterococcus faecalis]
TTCCATTTTAAATAGAATTATGCTATTATGAAAATGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACAC
CAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTT
ATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCGAAAATCAGTAGTGCA

Structures

Antitoxin