Detailed information of TA system TA223218

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 652652..652884
• Replicon: chromosome
• Accession: NZ_CP086411
• Organism: Enterococcus faecalis strain R5

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: -
• Locus tag: LMJ46_RS03355
• Protein ID: WP_033606579.1
• Coordinates: 652652..652768 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	652679..652884 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ46_RS03345 (648090)	648090..650348	+	2259	WP_002363179.1	DNA helicase PcrA	-
LMJ46_RS03350 (650474)	650474..652504	+	2031	WP_002389576.1	NAD-dependent DNA ligase LigA	-
LMJ46_RS03355 (652652)	652652..652768	+	117	WP_033606579.1	putative holin-like toxin	Toxin
- (652679)	652679..652884	-	206	NuclAT_4	-	Antitoxin
LMJ46_RS03360 (653086)	653086..653391	+	306	WP_002355569.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
LMJ46_RS03365 (653391)	653391..654860	+	1470	WP_002368026.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
LMJ46_RS03370 (654860)	654860..656290	+	1431	WP_002358703.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
LMJ46_RS03375 (656309)	656309..657397	+	1089	WP_002355572.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-35)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4073.99 Da
• Isoelectric Point: 5.9482

>T223218 WP_033606579.1 NZ_CP086411:652652-652768 [Enterococcus faecalis]
MLLSVEAALGLMISFAILVVTIIFGILALVLDNKNNRS

Nucleotide

• Length: 117 bp

>T223218 NZ_CP118590:2531772-2532050 [Vibrio harveyi]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTCTCTTATTGGGTCGAGGGCGATATCATCAGAGTTATG
CGTGTACTCCACCAGAAACAAAAATTCCCCACGGATTGA

Antitoxin

Nucleotide

• Length: 206 bp

>AT223218 NZ_CP086411:c652884-652679 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTATTGCAAAACTAATCATCAGTCC

Structures

Antitoxin