Detailed information of TA system TA223214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 98975..99702
• Replicon: plasmid pPOL9A
• Accession: NZ_CP086409
• Organism: Enterobacter cloacae strain POL9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LMJ45_RS24110
• Protein ID: WP_011251285.1
• Coordinates: 98975..99286 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LMJ45_RS24115
• Protein ID: WP_011251286.1
• Coordinates: 99283..99702 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ45_RS24075 (LMJ45_24075)	94289..95104	+	816	WP_064153610.1	hypothetical protein	-
LMJ45_RS24080 (LMJ45_24080)	95257..95721	+	465	WP_023338080.1	hypothetical protein	-
LMJ45_RS24085 (LMJ45_24085)	95733..96242	+	510	WP_064153612.1	hypothetical protein	-
LMJ45_RS24090 (LMJ45_24090)	96359..96514	-	156	WP_001352007.1	type I toxin-antitoxin system toxin HokD	-
LMJ45_RS24095 (LMJ45_24095)	96819..97595	-	777	Protein_76	RNA-guided endonuclease TnpB family protein	-
LMJ45_RS24105 (LMJ45_24105)	98363..98770	+	408	Protein_78	DDE-type integrase/transposase/recombinase	-
LMJ45_RS24110 (LMJ45_24110)	98975..99286	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LMJ45_RS24115 (LMJ45_24115)	99283..99702	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LMJ45_RS24120 (LMJ45_24120)	99928..100953	+	1026	WP_001101446.1	IS110 family transposase	-
LMJ45_RS24125 (LMJ45_24125)	101248..102216	+	969	WP_000654811.1	IS5 family transposase	-
LMJ45_RS24130 (LMJ45_24130)	102534..103559	-	1026	WP_001101446.1	IS110 family transposase	-
LMJ45_RS24135 (LMJ45_24135)	103644..103844	-	201	Protein_84	transposase	-
LMJ45_RS24140 (LMJ45_24140)	104126..104275	-	150	Protein_85	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108204	108204
	-	inside	IScluster/Tn	-	-	89481..103844	14363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T223214 WP_011251285.1 NZ_CP086409:98975-99286 [Enterobacter cloacae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T223214 NZ_CP118586:2516413-2516691 [Vibrio harveyi]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTCTCTTATTGGGTCGAGGGCGATATCATCAGAGTTATG
CGTGTACTCCACCAGAAACAAAAATTCCCCACGGATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT223214 WP_011251286.1 NZ_CP086409:99283-99702 [Enterobacter cloacae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT223214 NZ_CP118586:2516132-2516416 [Vibrio harveyi]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGAAC
TCTAAGTGATGCTTGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTGTTTTCGTTGAGCACCAAACTGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1