Detailed information of TA system TA223213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4873218..4873820
• Replicon: chromosome
• Accession: NZ_CP086408
• Organism: Enterobacter cloacae strain POL9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1AN02
• Locus tag: LMJ45_RS23305
• Protein ID: WP_013099360.1
• Coordinates: 4873509..4873820 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LMJ45_RS23300
• Protein ID: WP_128312298.1
• Coordinates: 4873218..4873508 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ45_RS23285 (LMJ45_23285)	4870715..4871617	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
LMJ45_RS23290 (LMJ45_23290)	4871614..4872249	+	636	WP_128312300.1	formate dehydrogenase cytochrome b556 subunit	-
LMJ45_RS23295 (LMJ45_23295)	4872246..4873175	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
LMJ45_RS23300 (LMJ45_23300)	4873218..4873508	-	291	WP_128312298.1	helix-turn-helix domain-containing protein	Antitoxin
LMJ45_RS23305 (LMJ45_23305)	4873509..4873820	-	312	WP_013099360.1	hypothetical protein	Toxin
LMJ45_RS23310 (LMJ45_23310)	4874050..4874175	+	126	Protein_4551	alpha/beta hydrolase	-
LMJ45_RS23315 (LMJ45_23315)	4874254..4875195	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
LMJ45_RS23320 (LMJ45_23320)	4875240..4875677	-	438	WP_013099363.1	D-aminoacyl-tRNA deacylase	-
LMJ45_RS23325 (LMJ45_23325)	4875674..4876555	-	882	WP_013099364.1	virulence factor BrkB family protein	-
LMJ45_RS23330 (LMJ45_23330)	4876549..4877148	-	600	WP_013099365.1	glucose-1-phosphatase	-
LMJ45_RS23335 (LMJ45_23335)	4877268..4878068	-	801	WP_020686879.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12210.18 Da
• Isoelectric Point: 9.7550

>T223213 WP_013099360.1 NZ_CP086408:c4873820-4873509 [Enterobacter cloacae]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHRTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T223213 NZ_CP118586:c2505890-2505588 [Vibrio harveyi]
ATGGCTGAAATAATCTGGACTGAGCCAGCGTTATCTGACCTCAACGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAGCAGTTAGTGCAAACGATCTTCTCCAAAGTCGAACGTTTACAAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGATCTAAAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTTTGTTTGTTATGCGCGCAGAGAGAGGTTTGCGTAAGTTCTTGTTAAATAAGCAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10637.15 Da
• Isoelectric Point: 10.4747

>AT223213 WP_128312298.1 NZ_CP086408:c4873508-4873218 [Enterobacter cloacae]
MDKALFERLTQSIAQMDDIIGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT223213 NZ_CP118586:c2506135-2505878 [Vibrio harveyi]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATTCTCGCCGATCTCCACGACACCAAAGAACCAGT
GTTAATTACTGAGCATGGTAAGCCATCCGCGTATCTCGTTGATGTTGATGACTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGACGGTAAAGTGGTAAGTCATGATGAAGCCAAGGGCAAAATG
TCAAAATGGCTGAAATAA

Structures

Toxin

• AlphaFold DB: A0A5E1AN02