Detailed information of TA system TA223175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4251081..4251683
• Replicon: chromosome
• Accession: NZ_CP086391
• Organism: Escherichia coli strain 1585m1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LM401_RS22690
• Protein ID: WP_000897305.1
• Coordinates: 4251372..4251683 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LM401_RS22685
• Protein ID: WP_000356397.1
• Coordinates: 4251081..4251371 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM401_RS22660 (4247006)	4247006..4247908	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LM401_RS22665 (4247905)	4247905..4248540	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LM401_RS22670 (4248537)	4248537..4249466	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LM401_RS22675 (4249796)	4249796..4250038	-	243	WP_001087409.1	protein YiiF	-
LM401_RS22680 (4250258)	4250258..4250476	-	219	WP_001315930.1	CopG family transcriptional regulator	-
LM401_RS22685 (4251081)	4251081..4251371	-	291	WP_000356397.1	NadS family protein	Antitoxin
LM401_RS22690 (4251372)	4251372..4251683	-	312	WP_000897305.1	hypothetical protein	Toxin
LM401_RS22695 (4251912)	4251912..4252820	+	909	WP_001162704.1	alpha/beta hydrolase	-
LM401_RS22700 (4252884)	4252884..4253825	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LM401_RS22705 (4253870)	4253870..4254307	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LM401_RS22710 (4254304)	4254304..4255176	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LM401_RS22715 (4255170)	4255170..4255769	-	600	WP_001295269.1	glucose-1-phosphatase	-
LM401_RS22720 (4255868)	4255868..4256653	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T223175 WP_000897305.1 NZ_CP086391:c4251683-4251372 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T223175 NZ_CP118544:532314-532664 [Brevibacillus parabrevis]
GTGATTGTCAAACGTGGCGATGTATTTTTCGCGGACCTGTCACCGGTCGTTGGCTCTGAGCAAGGAGGCGTGCGGCCTGT
TTTAGTCATTCAGAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCAGCCATTACGGCGCAAATTCAGAAGG
CAAAGCTGCCTACGCATGTGGAAATTGACGCAAAAACATATGGATTTGATCGTGACTCCGTCATTTTGTTGGAACAAATA
CGAACCATTGACAAACAAAGGCTGACCGATAAGATTACCCATCTCGATGACGAGATGATGGATCGGGTCAATGAGTCTTT
ACAAATCAGCTTGGGCCTCATTGATTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT223175 WP_000356397.1 NZ_CP086391:c4251371-4251081 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT223175 NZ_CP118544:532011-532310 [Brevibacillus parabrevis]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGTTTGCCGCAACACTTGCTACAGGA
AGTGGATGGCGTCATTCAAAAGGAGAAATCCAATCGCAGCGAGTTTATTCGTCAGGCGATGCATTTGTATTTGAAGGAGC
GTAAAAAACGTACGATCCGGGAGACAATGCAAAAAGGGTATATGGAGATGGCGAAGATTAATCTGAAAATGGCATCGGAG
GCTTTTGGCGCTGAGGAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7