Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3412049..3412590 Replicon chromosome
Accession NZ_CP086391
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LM401_RS18805 Protein ID WP_000615976.1
Coordinates 3412312..3412590 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LM401_RS18800 Protein ID WP_000729704.1
Coordinates 3412049..3412309 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS18775 (3407832) 3407832..3408617 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
LM401_RS18780 (3408589) 3408589..3410301 + 1713 Protein_3248 flagellar biosynthesis protein FlhA -
LM401_RS18785 (3410406) 3410406..3410684 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LM401_RS18790 (3410677) 3410677..3411033 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
LM401_RS18795 (3411090) 3411090..3411863 - 774 WP_000543895.1 C40 family peptidase -
LM401_RS18800 (3412049) 3412049..3412309 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LM401_RS18805 (3412312) 3412312..3412590 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LM401_RS18810 (3412746) 3412746..3413486 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LM401_RS18815 (3413457) 3413457..3414224 - 768 WP_000333380.1 class II glutamine amidotransferase -
LM401_RS18820 (3414430) 3414430..3415008 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3410677..3423376 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T223172 WP_000615976.1 NZ_CP086391:3412312-3412590 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T223172 NZ_CP118542:4461495-4461806 [Salmonella sp. CVCC 1806]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACACTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT223172 WP_000729704.1 NZ_CP086391:3412049-3412309 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT223172 NZ_CP118542:4461803-4462249 [Salmonella sp. CVCC 1806]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAATCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References