Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 265629..266428 Replicon chromosome
Accession NZ_CP086391
Organism Escherichia coli strain 1585m1

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag LM401_RS03550 Protein ID WP_000347266.1
Coordinates 265629..266093 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LM401_RS03555 Protein ID WP_001307405.1
Coordinates 266093..266428 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM401_RS03520 (260630) 260630..261064 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LM401_RS03525 (261082) 261082..261960 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
LM401_RS03530 (261950) 261950..262729 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LM401_RS03535 (262740) 262740..263213 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LM401_RS03540 (263236) 263236..264516 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
LM401_RS03545 (264765) 264765..265574 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LM401_RS03550 (265629) 265629..266093 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LM401_RS03555 (266093) 266093..266428 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LM401_RS03560 (266577) 266577..268148 - 1572 WP_001273741.1 galactarate dehydratase -
LM401_RS03565 (268523) 268523..269857 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LM401_RS03570 (269873) 269873..270643 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T223153 WP_000347266.1 NZ_CP086391:c266093-265629 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T223153 NZ_CP118539:c2530752-2530444 [Xylella fastidiosa]
GTGACCATAACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTTGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCCCTTCGGCCTGGTAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223153 WP_001307405.1 NZ_CP086391:c266428-266093 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223153 NZ_CP118539:c2531055-2530756 [Xylella fastidiosa]
ATGACCTACACGCTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCGGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References