Detailed information of TA system TA223149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3302..4029
• Replicon: plasmid p1585m1_B
• Accession: NZ_CP086388
• Organism: Escherichia coli strain 1585m1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: LM401_RS00600
• Protein ID: WP_000558568.1
• Coordinates: 3302..3613 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: LM401_RS00605
• Protein ID: WP_000990392.1
• Coordinates: 3610..4029 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM401_RS00570 (LM401_00570)	41..265	+	225	WP_229014371.1	hypothetical protein	-
LM401_RS00575 (LM401_00575)	277..981	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LM401_RS00580 (LM401_00580)	1117..1359	+	243	Protein_2	ISNCY family transposase	-
LM401_RS00585 (LM401_00585)	1408..1635	+	228	WP_181727888.1	helix-turn-helix domain-containing protein	-
LM401_RS00590 (LM401_00590)	1676..2644	-	969	WP_000654811.1	IS5 family transposase	-
LM401_RS00595 (LM401_00595)	2677..3072	+	396	Protein_5	Tn3 family transposase	-
LM401_RS00600 (LM401_00600)	3302..3613	+	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
LM401_RS00605 (LM401_00605)	3610..4029	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
LM401_RS00610 (LM401_00610)	4066..4890	-	825	WP_001141174.1	hypothetical protein	-
LM401_RS00615 (LM401_00615)	5015..5719	-	705	WP_061872725.1	IS6-like element IS26 family transposase	-
LM401_RS00620 (LM401_00620)	5783..5989	+	207	Protein_10	arsenate reductase	-
LM401_RS00625 (LM401_00625)	6020..6394	-	375	WP_000110555.1	ArsA-related P-loop ATPase	-
LM401_RS00630 (LM401_00630)	6433..7005	-	573	WP_001556711.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / catB3 / blaIMP-4	-	1..113047	113047
	-	inside	IScluster/Tn	-	-	277..10177	9900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T223149 WP_000558568.1 NZ_CP086388:3302-3613 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T223149 NZ_CP118539:1204182-1204448 [Xylella fastidiosa]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTAGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT223149 WP_000990392.1 NZ_CP086388:3610-4029 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT223149 NZ_CP118539:1203971-1204198 [Xylella fastidiosa]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTGAACTCTCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTACGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAACGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGATGTGAGGAAAACGCTTGGCCTGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4