Detailed information of TA system TA022312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1842680..1843272
• Replicon: chromosome
• Accession: NC_009663
• Organism: Sulfurovum sp. NBC37-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A6QB81
• Locus tag: SUN_RS09250
• Protein ID: WP_012083551.1
• Coordinates: 1842991..1843272 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SUN_RS09245
• Protein ID: WP_012083550.1
• Coordinates: 1842680..1842976 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SUN_RS09215	1837936..1838277	-	342	WP_012083544.1	hypothetical protein	-
SUN_RS09220	1838316..1839011	-	696	WP_012083545.1	HesA/MoeB/ThiF family protein	-
SUN_RS09225	1839023..1839844	-	822	WP_012083546.1	carbon-nitrogen hydrolase family protein	-
SUN_RS09230	1839844..1840056	-	213	WP_012083547.1	exodeoxyribonuclease VII small subunit	-
SUN_RS09235	1840114..1841211	-	1098	WP_012083548.1	homoserine O-acetyltransferase	-
SUN_RS09240	1841369..1842631	-	1263	WP_012083549.1	O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase	-
SUN_RS09245	1842680..1842976	-	297	WP_012083550.1	HigA family addiction module antidote protein	Antitoxin
SUN_RS09250	1842991..1843272	-	282	WP_012083551.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SUN_RS09255	1843378..1844823	-	1446	WP_012083552.1	IMP dehydrogenase	-
SUN_RS09260	1844842..1846185	-	1344	WP_012083553.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1842680..1851271	8591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11221.97 Da
• Isoelectric Point: 9.6119

>T22312 WP_012083551.1 NC_009663:c1843272-1842991 [Sulfurovum sp. NBC37-1]
MIKTFKCKETYKVYKREFSKKLQQDIQRTAYRKLLMIEASIDIDDLKIPPANRLEKLSGNREDQYSIRINKQYRICFRWE
SGSALDVEIVDYH

Nucleotide

• Length: 282 bp

>T22312 NC_009663:c1843272-1842991 [Sulfurovum sp. NBC37-1]
ATGATCAAAACATTTAAGTGTAAAGAGACTTACAAAGTTTATAAGCGTGAGTTCTCTAAAAAGCTGCAACAAGACATTCA
AAGAACAGCCTATAGGAAATTACTTATGATAGAGGCTTCTATAGATATTGATGACTTGAAAATACCACCTGCAAACAGGT
TGGAAAAACTTTCTGGAAATAGAGAAGATCAATACAGTATACGTATCAATAAACAGTATCGTATCTGTTTCCGATGGGAA
AGTGGCTCGGCTTTGGATGTTGAAATAGTAGATTATCATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11233.92 Da
• Isoelectric Point: 5.7202

>AT22312 WP_012083550.1 NC_009663:c1842976-1842680 [Sulfurovum sp. NBC37-1]
MKKITPIHAGEILLEEFMQPLGLSQNALAKALHITPRRINEIVNGKRSITADTALRLARFFGNSAEFWMNLQNKYDLEIA
RDILSEKIDEEVEFYQAS

Nucleotide

• Length: 297 bp

>AT22312 NC_009663:c1842976-1842680 [Sulfurovum sp. NBC37-1]
ATGAAGAAAATAACACCTATACATGCAGGAGAGATTCTTTTGGAAGAGTTCATGCAGCCTTTGGGTCTTAGCCAGAATGC
TTTGGCAAAGGCATTACATATAACTCCGAGACGGATCAATGAGATTGTTAATGGTAAAAGATCGATTACAGCAGATACCG
CATTGCGACTTGCAAGGTTTTTTGGAAACAGTGCTGAATTCTGGATGAATTTACAAAATAAATATGACCTTGAGATAGCA
CGTGATATTTTATCTGAGAAGATAGATGAAGAAGTAGAATTTTATCAAGCATCATAA

Structures

Toxin

• AlphaFold DB: A6QB81