Detailed information of TA system TA223118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3061792..3062516
• Replicon: chromosome
• Accession: NZ_CP086369
• Organism: Pectobacterium versatile strain A73-S18-O15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A093VC08
• Locus tag: LLE50_RS13880
• Protein ID: WP_039482109.1
• Coordinates: 3061792..3062103 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LLE50_RS13885
• Protein ID: WP_103861592.1
• Coordinates: 3062100..3062516 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE50_RS13850 (LLE50_13845)	3057157..3058221	+	1065	WP_130621307.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
LLE50_RS13855 (LLE50_13850)	3058231..3058965	+	735	WP_103971370.1	type I-E CRISPR-associated protein Cas5/CasD	-
LLE50_RS13860 (LLE50_13855)	3058965..3059612	+	648	WP_116362408.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
LLE50_RS13865 (LLE50_13860)	3059640..3060557	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1e	-
LLE50_RS13870 (LLE50_13865)	3060558..3060851	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
LLE50_RS13875 (LLE50_13870)	3061333..3061509	+	177	WP_180784025.1	hypothetical protein	-
LLE50_RS13880 (LLE50_13875)	3061792..3062103	+	312	WP_039482109.1	type II toxin-antitoxin system HigB family toxin	Toxin
LLE50_RS13885 (LLE50_13880)	3062100..3062516	+	417	WP_103861592.1	helix-turn-helix domain-containing protein	Antitoxin
LLE50_RS13890 (LLE50_13885)	3062736..3063239	-	504	WP_109224920.1	hypothetical protein	-
LLE50_RS13895 (LLE50_13890)	3063587..3063814	+	228	WP_226306457.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
LLE50_RS13900 (LLE50_13895)	3064851..3065309	+	459	WP_039470406.1	hypothetical protein	-
LLE50_RS13905 (LLE50_13900)	3065407..3066759	-	1353	WP_130621306.1	pyridoxal-dependent decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3053978..3063814	9836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.29 Da
• Isoelectric Point: 9.8530

>T223118 WP_039482109.1 NZ_CP086369:3061792-3062103 [Pectobacterium versatile]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T223118 NZ_CP118532:1751563-1751865 [Vibrio harveyi]
ATGGCTGAAATAATCTGGACTGAGCCAGCGTTATCTGACCTCAACGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAGCAGTTAGTGCAAACGATCTTCTCCAAAGTCGAACGTTTACAAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGATCTAAAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTTTGTTTGTTATGCGCGCAGAGAGAGGTTTGCGTAAGTTCTTGTTAAATAAGCAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15405.70 Da
• Isoelectric Point: 4.8037

>AT223118 WP_103861592.1 NZ_CP086369:3062100-3062516 [Pectobacterium versatile]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT223118 NZ_CP118532:1751318-1751575 [Vibrio harveyi]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATTCTCGCCGATCTCCACGACACCAAAGAACCAGT
GTTAATTACTGAGCATGGTAAGCCATCCGCGTATCTCGTTGATGTTGATGACTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGACGGTAAAGTGGTAAGTCATGATGAAGCCAAGGTCAAAATG
TCAAAATGGCTGAAATAA

Structures

Toxin

• AlphaFold DB: A0A093VC08