Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4112851..4113578 Replicon chromosome
Accession NZ_CP086342
Organism Escherichia coli strain ESY001

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C5N06_RS19775 Protein ID WP_000550189.1
Coordinates 4113264..4113578 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C5N06_RS19770 Protein ID WP_000560266.1
Coordinates 4112851..4113267 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N06_RS19760 (4108011) 4108011..4110362 + 2352 WP_000695487.1 alpha-glucosidase -
C5N06_RS19765 (4110788) 4110788..4112806 + 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C5N06_RS19770 (4112851) 4112851..4113267 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C5N06_RS19775 (4113264) 4113264..4113578 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C5N06_RS19780 (4113862) 4113862..4114998 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C5N06_RS19785 (4115083) 4115083..4115586 + 504 WP_001333820.1 M48 family metallopeptidase -
C5N06_RS19790 (4115663) 4115663..4116355 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
C5N06_RS19795 (4116434) 4116434..4117420 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T223099 WP_000550189.1 NZ_CP086342:c4113578-4113264 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T223099 NZ_CP118442:c4451428-4450109 [Aeromonas caviae]
ATGGCCAATCTGGCTGTCTATATGAACGGCTATCGGGTAGGCACATTCACCAAAACTACCAGCGGCGCCCATCATTTTCA
GTACCATGAGTCATGGCTAGCACAACCGGGCAGCCGTCCGATCTCACTCTCCATGCCATTGCGCCATCAGACCTACCGGG
GCGATGAAGCCTATAACTTCTTCGACAACCTGCTGCCAGATAATACGGAGGTGAGAAACAGGGTTGTAGCACGCTATCAA
GCGGCATCAACCCAGCCCTTCGATCTGCTCAGTTGCATTGGCCAGGATAGCGTAGGGGCATTACAGCTGGTTACCGAAGG
GCACGATGTTCCAGATGTCAGGCGAATCGACTACAAGGCACTTTCCGATGACGAGCTCGAGCAGATCCTGACCAGCTACA
AATCAGGAATCCCTTTGGGGATGGTCAGGGAGCAAGAGGAGTTCCGGATCTCTATCGCCGGGGCGCAAGAAAAGACTGCA
CTGCTCTATCTCGATGGCCGTTGGTACTTGCCACTCAATGCAACGCCGACAACCCACATAATCAAACTCCCTATCGGCAA
GATTGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACTCTGATTGCCAAGGAGT
TTGGTCTACCCGTACCTCACTGCTTCATGATGCAGGTTGGCCAGGTCAAAGCACTGGCTGTGGAACGCTTCGACAGACGT
TACGCTGCCGATCGCAGTTGGATCATGCGCCTTCCCCAAGAAGATTTCTGTCAGGTATTGAATGTTCCGTCAGCACGGAA
ATACGAGAACCATGGAGGACCGGGGATCACTGACATCATGAAAACCTTGCTGGGCTCTGCGACACCAGAACAGGATCGCT
ACCTATTCATGAAATCTCAGGTCCTGTTCTGGCTGCTGGCGGCCACCGATGGCCATGCAAAAAACTTCTCGGTCTTTATT
GAGCCGGAAGGGCGTTTTCGCCTGACCCCTTTTTACGACATCCTATCTGTCTATCCAGTATTTGGCGGCCGAGGCCTCAA
CCGAAGGGACGCCAAGCTCGCTATGGGCCTAGCAAGCAGCAAGGGAAAGAAGTACGCCATTGAGCAGATATTCCCGCGTC
ATTTCTTCCAAACGGCGAAAGCTGTGGGATTTGAGCGTTCTGCTATGGAAGAGATCCTGACAGAACTGGCCAACTCCGTT
GATGATGTTATTGAGCGCGTTACACAGCAATTGCCAGCAGGCTTCCCCGATACCATCAGCAGCACCATTCTAGAGGGCCT
AAAAGCCCGCTCAGCCAGGCTGATAAAGGGCTGGGATTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT223099 WP_000560266.1 NZ_CP086342:c4113267-4112851 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT223099 NZ_CP118442:c4451682-4451428 [Aeromonas caviae]
ATGAAAGTAACCAGCGCCAAGCAGCTGAGCGCCTGTATCAAGGATGCCAGGCTTTCTCAAAAGCTCTCTCAAGGCAAAGT
GGCCGAGAAAGTAGGGATTAGGCAAGACACAGTCTCCAGCTTCGAGCTCAACCCTGAGTCCACCAAGCTGGAGACCCTGT
TCAAGATCCTCGCGGCACTCGAGTTGTCAGTGGATATCAAACCCCGCAATGAGTCATCCGAGAGTCCGGCATCGGGCTGG
AAGGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References