Detailed information of TA system TA223081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1014948..1015642
• Replicon: chromosome
• Accession: NZ_CP086342
• Organism: Escherichia coli strain ESY001

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: C5N06_RS04835
• Protein ID: WP_001263489.1
• Coordinates: 1015244..1015642 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: C5N06_RS04830
• Protein ID: WP_000554758.1
• Coordinates: 1014948..1015241 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N06_RS04810 (1010580)	1010580..1011077	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
C5N06_RS04815 (1011301)	1011301..1013013	-	1713	Protein_924	flagellar biosynthesis protein FlhA	-
C5N06_RS04820 (1012985)	1012985..1013770	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
C5N06_RS04825 (1013841)	1013841..1014896	+	1056	WP_001226164.1	DNA polymerase IV	-
C5N06_RS04830 (1014948)	1014948..1015241	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C5N06_RS04835 (1015244)	1015244..1015642	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C5N06_RS04840 (1015652)	1015652..1016104	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
C5N06_RS04845 (1016422)	1016422..1016628	+	207	Protein_930	RtcB family protein	-
C5N06_RS04850 (1016624)	1016624..1017145	+	522	Protein_931	peptide chain release factor H	-
C5N06_RS04855 (1017202)	1017202..1018659	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
C5N06_RS04860 (1018920)	1018920..1019378	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (1019974)	1019974..1020054	+	81	NuclAT_11	-	-
- (1019974)	1019974..1020054	+	81	NuclAT_11	-	-
- (1019974)	1019974..1020054	+	81	NuclAT_11	-	-
- (1019974)	1019974..1020054	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T223081 WP_001263489.1 NZ_CP086342:1015244-1015642 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T223081 NZ_CP118399:16851-17267 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT223081 WP_000554758.1 NZ_CP086342:1014948-1015241 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT223081 NZ_CP118399:16624-16854 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3