223073

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3757009..3757550 Replicon chromosome
Accession NZ_CP086340
Organism Escherichia coli strain ESY003

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag C5N08_RS18145 Protein ID WP_000615983.1
Coordinates 3757272..3757550 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag C5N08_RS18140 Protein ID WP_000729703.1
Coordinates 3757009..3757269 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N08_RS18120 (3752684) 3752684..3753469 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
C5N08_RS18125 (3753441) 3753441..3755153 + 1713 Protein_3550 flagellar biosynthesis protein FlhA -
C5N08_RS18130 (3755377) 3755377..3755874 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
C5N08_RS18135 (3756050) 3756050..3756799 - 750 WP_000056849.1 C40 family peptidase -
C5N08_RS18140 (3757009) 3757009..3757269 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C5N08_RS18145 (3757272) 3757272..3757550 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C5N08_RS18150 (3757706) 3757706..3758446 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
C5N08_RS18155 (3758417) 3758417..3759184 - 768 WP_000333380.1 class II glutamine amidotransferase -
C5N08_RS18160 (3759390) 3759390..3759968 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T223073 WP_000615983.1 NZ_CP086340:3757272-3757550 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T223073 NZ_CP118398:c3829487-3829170 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT223073 WP_000729703.1 NZ_CP086340:3757009-3757269 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT223073 NZ_CP118398:c3829177-3828890 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References