Detailed information of TA system TA022306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5541534..5542129
• Replicon: chromosome
• Accession: NC_009656
• Organism: Pseudomonas aeruginosa PA7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PSPA7_RS31625
• Protein ID: WP_003113526.1
• Coordinates: 5541851..5542129 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PSPA7_RS25670
• Protein ID: WP_012077435.1
• Coordinates: 5541534..5541839 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSPA7_RS25650	5536625..5536912	-	288	WP_041025529.1	DUF5447 family protein	-
PSPA7_RS25655	5536939..5537211	-	273	WP_004352675.1	hypothetical protein	-
PSPA7_RS31615	5537832..5538164	+	333	WP_071535397.1	hypothetical protein	-
PSPA7_RS32240	5538219..5538884	-	666	WP_138009465.1	hypothetical protein	-
PSPA7_RS31620	5538921..5540252	-	1332	WP_012077433.1	AAA family ATPase	-
PSPA7_RS25665	5540290..5541138	-	849	WP_041025421.1	restriction endonuclease	-
PSPA7_RS25670	5541534..5541839	-	306	WP_012077435.1	HigA family addiction module antidote protein	Antitoxin
PSPA7_RS31625	5541851..5542129	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSPA7_RS25675	5542800..5543003	+	204	WP_012077436.1	hypothetical protein	-
PSPA7_RS25680	5543246..5545105	+	1860	WP_012077437.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T22306 WP_003113526.1 NC_009656:c5542129-5541851 [Pseudomonas aeruginosa PA7]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T22306 NC_009656:c5542129-5541851 [Pseudomonas aeruginosa PA7]
ATGATTCTGACCTTTCGATGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCCATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCTGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACAGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11184.65 Da
• Isoelectric Point: 4.6081

>AT22306 WP_012077435.1 NC_009656:c5541839-5541534 [Pseudomonas aeruginosa PA7]
MATNGMRPIHPGEILRDEFLTELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT22306 NC_009656:c5541839-5541534 [Pseudomonas aeruginosa PA7]
ATGGCTACCAATGGTATGCGCCCTATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGACGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCCCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3