Detailed information of TA system TA223059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1855455..1855957
• Replicon: chromosome
• Accession: NZ_CP086340
• Organism: Escherichia coli strain ESY003

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: C5N08_RS08780
• Protein ID: WP_000767829.1
• Coordinates: 1855703..1855957 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: C5N08_RS08775
• Protein ID: WP_001259255.1
• Coordinates: 1855455..1855706 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N08_RS08750 (1850632)	1850632..1851699	-	1068	WP_046464156.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
C5N08_RS08755 (1851699)	1851699..1852769	-	1071	WP_000108945.1	histidinol-phosphate transaminase	-
C5N08_RS08760 (1852766)	1852766..1854070	-	1305	WP_001446927.1	histidinol dehydrogenase	-
C5N08_RS08765 (1854076)	1854076..1854975	-	900	WP_032329340.1	ATP phosphoribosyltransferase	-
C5N08_RS08770 (1855121)	1855121..1855171	-	51	WP_001364200.1	his operon leader peptide	-
C5N08_RS08775 (1855455)	1855455..1855706	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
C5N08_RS08780 (1855703)	1855703..1855957	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
C5N08_RS08785 (1856040)	1856040..1856864	+	825	WP_000754749.1	SDR family oxidoreductase	-
C5N08_RS08790 (1856910)	1856910..1857839	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
C5N08_RS08795 (1858054)	1858054..1858116	+	63	WP_010723108.1	membrane protein YoeI	-
C5N08_RS08800 (1858106)	1858106..1859464	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
C5N08_RS08805 (1859643)	1859643..1860701	+	1059	WP_000492321.1	thiosulfate utilization transporter TsuA/YeeE	-
C5N08_RS08810 (1860715)	1860715..1860942	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T223059 WP_000767829.1 NZ_CP086340:1855703-1855957 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T223059 NZ_CP118384:69167-69454 [Enterobacter hormaechei]
ATGGCATATTTTCTGGATTTTGACGAGCGTGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGCTAAA
AAATAAGTTGGCTGAAGTGCTTGAGTCACCCCGAATTGATGCGAACAAGCTTCGGGGTATGCATGACTGTTACAAAATTA
AGCTCCGGTCTTCAGGCTATCGTCTTGTATATCAGGTCATAGACGAGAAGGTTGTCGTCTTTGTGATTTCTGTCGGGAAA
AGAGAGCGCTCGGAAGTTTATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT223059 WP_001259255.1 NZ_CP086340:1855455-1855706 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT223059 NZ_CP118384:68928-69167 [Enterobacter hormaechei]
ATGGGTAGCATCAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCTGCGCTTGAAAAAATGGGTGTAACTCC
CTCTGAGGCACTTCGCCTCATGCTTGAATATATTGCTGACAATGAACGTTTGCCGTTCAAACAGACACTCCTGAGTGATG
AGGATGCTGAGCTCGTGGAGATCGTGAAAGAACGACTTCGCAATCCTAAGCCAGTACGTGTGACGCTGGATGAGCTCTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0