Detailed information of TA system TA223055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 721925..722618
• Replicon: chromosome
• Accession: NZ_CP086340
• Organism: Escherichia coli strain ESY003

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: C5N08_RS03525
• Protein ID: WP_000415584.1
• Coordinates: 721925..722221 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: C5N08_RS03530
• Protein ID: WP_000650107.1
• Coordinates: 722223..722618 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N08_RS03490 (717013)	717013..717327	-	315	WP_000958598.1	putative quinol monooxygenase	-
C5N08_RS03495 (717358)	717358..717939	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
C5N08_RS03500 (718258)	718258..718590	+	333	WP_000917685.1	DUF2645 family protein	-
C5N08_RS03505 (718636)	718636..719985	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
C5N08_RS03510 (719982)	719982..720641	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
C5N08_RS03515 (720793)	720793..721185	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
C5N08_RS03520 (721238)	721238..721720	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
C5N08_RS03525 (721925)	721925..722221	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
C5N08_RS03530 (722223)	722223..722618	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
C5N08_RS03535 (722751)	722751..724358	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
C5N08_RS03540 (724496)	724496..726754	+	2259	WP_032329482.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T223055 WP_000415584.1 NZ_CP086340:721925-722221 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T223055 NZ_CP118383:c3726066-3725779 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGTGTTATTGTTATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT223055 WP_000650107.1 NZ_CP086340:722223-722618 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT223055 NZ_CP118383:c3725792-3725490 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65