Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3960735..3961534 Replicon chromosome
Accession NZ_CP086338
Organism Escherichia coli strain EJN001

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag C5N09_RS19175 Protein ID WP_000347275.1
Coordinates 3961070..3961534 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C5N09_RS19170 Protein ID WP_001307405.1
Coordinates 3960735..3961070 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N09_RS19155 (3956520) 3956520..3957290 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
C5N09_RS19160 (3957306) 3957306..3958640 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
C5N09_RS19165 (3959015) 3959015..3960586 + 1572 WP_001273752.1 galactarate dehydratase -
C5N09_RS19170 (3960735) 3960735..3961070 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C5N09_RS19175 (3961070) 3961070..3961534 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C5N09_RS19180 (3961589) 3961589..3962398 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C5N09_RS19185 (3962647) 3962647..3963927 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
C5N09_RS19190 (3963950) 3963950..3964423 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
C5N09_RS19195 (3964434) 3964434..3965213 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
C5N09_RS19200 (3965203) 3965203..3966081 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
C5N09_RS19205 (3966099) 3966099..3966533 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T223052 WP_000347275.1 NZ_CP086338:3961070-3961534 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T223052 NZ_CP118383:c579532-579314 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223052 WP_001307405.1 NZ_CP086338:3960735-3961070 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223052 NZ_CP118383:c579934-579560 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References