Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3935028..3935755 Replicon chromosome
Accession NZ_CP086338
Organism Escherichia coli strain EJN001

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C5N09_RS19035 Protein ID WP_000550189.1
Coordinates 3935441..3935755 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C5N09_RS19030 Protein ID WP_000560266.1
Coordinates 3935028..3935444 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N09_RS19020 (3930188) 3930188..3932539 + 2352 WP_000695487.1 alpha-glucosidase -
C5N09_RS19025 (3932965) 3932965..3934983 + 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C5N09_RS19030 (3935028) 3935028..3935444 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C5N09_RS19035 (3935441) 3935441..3935755 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C5N09_RS19040 (3936039) 3936039..3937175 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C5N09_RS19045 (3937260) 3937260..3937763 + 504 WP_001333820.1 M48 family metallopeptidase -
C5N09_RS19050 (3937840) 3937840..3938532 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
C5N09_RS19055 (3938611) 3938611..3939597 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T223051 WP_000550189.1 NZ_CP086338:c3935755-3935441 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T223051 NZ_CP118332:c4562799-4562458 [Klebsiella aerogenes]
ATGAAAACTCTACCTGCAACAACGCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGTAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT223051 WP_000560266.1 NZ_CP086338:c3935444-3935028 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT223051 NZ_CP118332:c4563161-4562820 [Klebsiella aerogenes]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCCAGTGGGGGCTGAATCGTAATGTGACGCCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGTTGACCGGGCCAGCATTACCGGTGACTTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CATTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References