Detailed information of TA system TA223033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 897403..898097
• Replicon: chromosome
• Accession: NZ_CP086338
• Organism: Escherichia coli strain EJN001

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: C5N09_RS04300
• Protein ID: WP_001263493.1
• Coordinates: 897699..898097 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: C5N09_RS04295
• Protein ID: WP_001399937.1
• Coordinates: 897403..897696 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N09_RS04270 (892667)	892667..892765	+	99	Protein_827	peptidoglycan endopeptidase	-
C5N09_RS04275 (892941)	892941..893438	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
C5N09_RS04280 (893756)	893756..895468	-	1713	Protein_829	flagellar biosynthesis protein FlhA	-
C5N09_RS04285 (895440)	895440..896225	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
C5N09_RS04290 (896296)	896296..897351	+	1056	WP_001226161.1	DNA polymerase IV	-
C5N09_RS04295 (897403)	897403..897696	+	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C5N09_RS04300 (897699)	897699..898097	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C5N09_RS04305 (898107)	898107..898559	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
C5N09_RS04310 (898878)	898878..899084	+	207	Protein_835	RtcB family protein	-
C5N09_RS04315 (899080)	899080..899601	+	522	Protein_836	peptide chain release factor H	-
C5N09_RS04320 (899658)	899658..901115	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
C5N09_RS04325 (901376)	901376..901834	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (902430)	902430..902510	+	81	NuclAT_13	-	-
- (902430)	902430..902510	+	81	NuclAT_13	-	-
- (902430)	902430..902510	+	81	NuclAT_13	-	-
- (902430)	902430..902510	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T223033 WP_001263493.1 NZ_CP086338:897699-898097 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T223033 NZ_CP118278:3936249-3936467 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT223033 WP_001399937.1 NZ_CP086338:897403-897696 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT223033 NZ_CP118278:3935848-3936222 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1EXB8