Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3966152..3966951 Replicon chromosome
Accession NZ_CP086336
Organism Escherichia coli strain EBJ001

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag C5N11_RS19180 Protein ID WP_000347275.1
Coordinates 3966487..3966951 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C5N11_RS19175 Protein ID WP_001307405.1
Coordinates 3966152..3966487 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N11_RS19160 (3961937) 3961937..3962707 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
C5N11_RS19165 (3962723) 3962723..3964057 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
C5N11_RS19170 (3964432) 3964432..3966003 + 1572 WP_001273752.1 galactarate dehydratase -
C5N11_RS19175 (3966152) 3966152..3966487 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C5N11_RS19180 (3966487) 3966487..3966951 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C5N11_RS19185 (3967006) 3967006..3967815 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C5N11_RS19190 (3968064) 3968064..3969344 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
C5N11_RS19195 (3969367) 3969367..3969840 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
C5N11_RS19200 (3969851) 3969851..3970630 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
C5N11_RS19205 (3970620) 3970620..3971498 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
C5N11_RS19210 (3971516) 3971516..3971950 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T223029 WP_000347275.1 NZ_CP086336:3966487-3966951 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T223029 NZ_CP118277:c4688152-4687871 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAATCTCCAGCAGATTATGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223029 WP_001307405.1 NZ_CP086336:3966152-3966487 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223029 NZ_CP118277:c4688384-4688142 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGTCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References