Detailed information of TA system TA223010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 898836..899530
• Replicon: chromosome
• Accession: NZ_CP086336
• Organism: Escherichia coli strain EBJ001

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: C5N11_RS04305
• Protein ID: WP_001263493.1
• Coordinates: 899132..899530 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: C5N11_RS04300
• Protein ID: WP_001399937.1
• Coordinates: 898836..899129 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N11_RS04275 (894100)	894100..894198	+	99	Protein_828	peptidoglycan endopeptidase	-
C5N11_RS04280 (894374)	894374..894871	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
C5N11_RS04285 (895189)	895189..896901	-	1713	Protein_830	flagellar biosynthesis protein FlhA	-
C5N11_RS04290 (896873)	896873..897658	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
C5N11_RS04295 (897729)	897729..898784	+	1056	WP_001226161.1	DNA polymerase IV	-
C5N11_RS04300 (898836)	898836..899129	+	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C5N11_RS04305 (899132)	899132..899530	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C5N11_RS04310 (899540)	899540..899992	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
C5N11_RS04315 (900311)	900311..900517	+	207	Protein_836	RtcB family protein	-
C5N11_RS04320 (900513)	900513..901034	+	522	Protein_837	peptide chain release factor H	-
C5N11_RS04325 (901091)	901091..902548	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
C5N11_RS04330 (902809)	902809..903267	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (903863)	903863..903943	+	81	NuclAT_13	-	-
- (903863)	903863..903943	+	81	NuclAT_13	-	-
- (903863)	903863..903943	+	81	NuclAT_13	-	-
- (903863)	903863..903943	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T223010 WP_001263493.1 NZ_CP086336:899132-899530 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T223010 NZ_CP118274:c4618045-4617671 [Klebsiella quasipneumoniae]
ATGCAAAAACAATCCCTGTCCTCTCATAGGGCGGCCTCGTCACGTCTGTCATCAATCGAAATCTGGCGAAAATTACTGAA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAGCACATTG
ATGCGGGAATCTCACTGTGTGATGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGACAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT223010 WP_001399937.1 NZ_CP086336:898836-899129 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT223010 NZ_CP118274:c4618454-4618095 [Klebsiella quasipneumoniae]
ATGAAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAACAGATGGAGCTTATGCTGGCCAGCAGAGAACTCACG
CCCCACATTCAGCGCTGCATTACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATCT
GTACATCGTAATTTATCCGACATCTGCAACAACCGCATAA

Structures

Toxin

• AlphaFold DB: S1EXB8