Detailed information of TA system TA223006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4566990..4567717
• Replicon: chromosome
• Accession: NZ_CP086334
• Organism: Escherichia coli strain EBJ003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: C5N12_RS22125
• Protein ID: WP_001521139.1
• Coordinates: 4566990..4567301 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5N12_RS22130
• Protein ID: WP_000126294.1
• Coordinates: 4567298..4567717 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N12_RS22100 (4562925)	4562925..4564634	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
C5N12_RS22105 (4564644)	4564644..4565186	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C5N12_RS22110 (4565186)	4565186..4565953	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
C5N12_RS22115 (4565950)	4565950..4566360	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C5N12_RS22120 (4566353)	4566353..4566823	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C5N12_RS22125 (4566990)	4566990..4567301	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
C5N12_RS22130 (4567298)	4567298..4567717	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C5N12_RS22135 (4567796)	4567796..4569220	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
C5N12_RS22140 (4569229)	4569229..4570686	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C5N12_RS22145 (4570946)	4570946..4571956	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
C5N12_RS22150 (4572105)	4572105..4572632	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T223006 WP_001521139.1 NZ_CP086334:4566990-4567301 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T223006 NZ_CP118274:802360-802770 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT223006 WP_000126294.1 NZ_CP086334:4567298-4567717 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT223006 NZ_CP118274:802113-802379 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9