Detailed information of TA system TA222999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3158633..3159235
• Replicon: chromosome
• Accession: NZ_CP086334
• Organism: Escherichia coli strain EBJ003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C5N12_RS15390
• Protein ID: WP_000897305.1
• Coordinates: 3158924..3159235 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5N12_RS15385
• Protein ID: WP_000356395.1
• Coordinates: 3158633..3158923 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N12_RS15350 (3154256)	3154256..3155158	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C5N12_RS15355 (3155155)	3155155..3155790	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C5N12_RS15360 (3155787)	3155787..3156716	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C5N12_RS15365 (3156898)	3156898..3157140	-	243	WP_021523315.1	hypothetical protein	-
C5N12_RS15370 (3157359)	3157359..3157577	-	219	WP_001295676.1	CopG family transcriptional regulator	-
C5N12_RS15375 (3157996)	3157996..3158274	-	279	WP_001306650.1	hypothetical protein	-
C5N12_RS15380 (3158336)	3158336..3158548	-	213	WP_000197769.1	hypothetical protein	-
C5N12_RS15385 (3158633)	3158633..3158923	-	291	WP_000356395.1	NadS family protein	Antitoxin
C5N12_RS15390 (3158924)	3158924..3159235	-	312	WP_000897305.1	hypothetical protein	Toxin
C5N12_RS15395 (3159464)	3159464..3160372	+	909	WP_001306651.1	alpha/beta hydrolase	-
C5N12_RS15400 (3160541)	3160541..3161455	-	915	WP_077634137.1	transposase	-
C5N12_RS15405 (3161468)	3161468..3162355	-	888	Protein_3011	hypothetical protein	-
C5N12_RS15410 (3162844)	3162844..3163302	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3162844..3163302	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T222999 WP_000897305.1 NZ_CP086334:c3159235-3158924 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T222999 NZ_CP118272:c4688152-4687871 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAATCTCCAGCAGATTATGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT222999 WP_000356395.1 NZ_CP086334:c3158923-3158633 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT222999 NZ_CP118272:c4688384-4688142 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGTCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7