Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 2244874..2245568 | Replicon | chromosome |
| Accession | NZ_CP086334 | ||
| Organism | Escherichia coli strain EBJ003 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | A0A0D8WHS4 |
| Locus tag | C5N12_RS11015 | Protein ID | WP_001521903.1 |
| Coordinates | 2244874..2245272 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | C5N12_RS11020 | Protein ID | WP_000554758.1 |
| Coordinates | 2245275..2245568 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (2240704) | 2240704..2240784 | - | 81 | NuclAT_11 | - | - |
| - (2240704) | 2240704..2240784 | - | 81 | NuclAT_11 | - | - |
| - (2240704) | 2240704..2240784 | - | 81 | NuclAT_11 | - | - |
| - (2240704) | 2240704..2240784 | - | 81 | NuclAT_11 | - | - |
| C5N12_RS10985 (2240044) | 2240044..2241288 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| C5N12_RS10990 (2241380) | 2241380..2241838 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| C5N12_RS10995 (2242099) | 2242099..2243556 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| C5N12_RS11000 (2243613) | 2243613..2243965 | - | 353 | Protein_2157 | peptide chain release factor H | - |
| C5N12_RS11005 (2243961) | 2243961..2244167 | - | 207 | Protein_2158 | RtcB family protein | - |
| C5N12_RS11010 (2244412) | 2244412..2244864 | - | 453 | WP_023144376.1 | GNAT family N-acetyltransferase | - |
| C5N12_RS11015 (2244874) | 2244874..2245272 | - | 399 | WP_001521903.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| C5N12_RS11020 (2245275) | 2245275..2245568 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| C5N12_RS11025 (2245620) | 2245620..2246675 | - | 1056 | WP_001226177.1 | DNA polymerase IV | - |
| C5N12_RS11030 (2246746) | 2246746..2247531 | - | 786 | WP_000207556.1 | putative lateral flagellar export/assembly protein LafU | - |
| C5N12_RS11035 (2247503) | 2247503..2249215 | + | 1713 | Protein_2164 | flagellar biosynthesis protein FlhA | - |
| C5N12_RS11040 (2249294) | 2249294..2249647 | + | 354 | WP_024166466.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| C5N12_RS11045 (2249704) | 2249704..2250453 | - | 750 | WP_016233951.1 | C40 family peptidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 2234811..2245568 | 10757 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15471.88 Da Isoelectric Point: 8.0949
>T222994 WP_001521903.1 NZ_CP086334:c2245272-2244874 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T222994 NZ_CP118272:802360-802770 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT222994 WP_000554758.1 NZ_CP086334:c2245568-2245275 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT222994 NZ_CP118272:802113-802379 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0D8WHS4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |