Detailed information of TA system TA222977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 107274..107776
• Replicon: chromosome
• Accession: NZ_CP086334
• Organism: Escherichia coli strain EBJ003

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: C5N12_RS00535
• Protein ID: WP_000767829.1
• Coordinates: 107522..107776 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: C5N12_RS00530
• Protein ID: WP_001259255.1
• Coordinates: 107274..107525 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N12_RS00505 (102452)	102452..103519	-	1068	WP_000080136.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
C5N12_RS00510 (103519)	103519..104589	-	1071	WP_000108937.1	histidinol-phosphate transaminase	-
C5N12_RS00515 (104586)	104586..105890	-	1305	WP_021523144.1	histidinol dehydrogenase	-
C5N12_RS00520 (105896)	105896..106795	-	900	WP_021523143.1	ATP phosphoribosyltransferase	-
C5N12_RS00525 (106941)	106941..106991	-	51	WP_001364200.1	his operon leader peptide	-
C5N12_RS00530 (107274)	107274..107525	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
C5N12_RS00535 (107522)	107522..107776	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
C5N12_RS00540 (107859)	107859..108683	+	825	WP_000754758.1	SDR family oxidoreductase	-
C5N12_RS00545 (108729)	108729..109658	+	930	WP_023154044.1	LysR substrate-binding domain-containing protein	-
C5N12_RS00550 (109873)	109873..109935	+	63	WP_010723108.1	membrane protein YoeI	-
C5N12_RS00555 (109925)	109925..111283	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
C5N12_RS00560 (111462)	111462..112520	+	1059	WP_000492324.1	thiosulfate utilization transporter TsuA/YeeE	-
C5N12_RS00565 (112534)	112534..112761	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T222977 WP_000767829.1 NZ_CP086334:107522-107776 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T222977 NZ_CP118233:c5025701-5025444 [Micromonospora chalcea]
GTGAGGCTGGTCTTCACCGCGACGGCCTGGAATCAGTACCTCAGCCACACCGACCGCAAGTTGGTCAAGCGCATCAACGA
CCTGATCACGGACGTGATGCGCAACGGATACGAGGGCATCGGCAAGCCGGAGCCCTTGCGGGGAGAGCTGTCCGGCTTCT
GGTCCCGCCGGATCGATCGCGAACACCGGCTGGTGTACCGGATCACCAAGGACGACGTCGAGATCATCGCCTGCCGCTAC
CACTACGGCGACCGCTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT222977 WP_001259255.1 NZ_CP086334:107274-107525 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT222977 NZ_CP118233:c5025973-5025698 [Micromonospora chalcea]
ATGCGGACCGTGAACTTCACCCAACTGCGCCAGAACCTAGCCGCCGAACTCGACAGCGTCATCAACGACGCCGAGGAAGT
GGTGGTGACCCGCTCGGGCCACGAACCGGTGGTCATCGTCCCGCTTGCCGAGTACGAGGCGATGAAGGAAACCGAATACC
TCCTGCGCAACCCGGCCAACGCCGCAGCCCTGCGCCGATCGATCGCCGAGCTTGAGCAGGGTGACGCGGCTGAGCGGGAC
CTCGTCGACCCGGCCACCGTGCGAGACGTCGCGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0