Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 204392..204914 Replicon chromosome
Accession NZ_CP086326
Organism Lacticaseibacillus rhamnosus strain PMC203

Toxin (Protein)


Gene name yoeB Uniprot ID C2JUK2
Locus tag LL671_RS00895 Protein ID WP_005687756.1
Coordinates 204392..204652 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C2JUK3
Locus tag LL671_RS00900 Protein ID WP_005687754.1
Coordinates 204645..204914 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL671_RS00870 (LL671_00870) 199808..200449 + 642 WP_005690763.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -
LL671_RS00875 (LL671_00875) 200517..201296 + 780 WP_005690765.1 DeoR/GlpR family DNA-binding transcription regulator -
LL671_RS00880 (LL671_00880) 201481..202368 + 888 WP_014571690.1 L-ribulose-5-phosphate 3-epimerase -
LL671_RS00885 (LL671_00885) 202435..203256 - 822 WP_005690770.1 Cof-type HAD-IIB family hydrolase -
LL671_RS00890 (LL671_00890) 203453..204181 + 729 WP_014571689.1 L-ribulose-5-phosphate 4-epimerase -
LL671_RS00895 (LL671_00895) 204392..204652 - 261 WP_005687756.1 Txe/YoeB family addiction module toxin Toxin
LL671_RS00900 (LL671_00900) 204645..204914 - 270 WP_005687754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LL671_RS00905 (LL671_00905) 205540..206340 + 801 WP_005690776.1 SDR family oxidoreductase -
LL671_RS00910 (LL671_00910) 206367..208235 + 1869 WP_014571687.1 HTH domain-containing protein -
LL671_RS00915 (LL671_00915) 208236..208745 + 510 WP_049179682.1 transcriptional regulator GutM -
LL671_RS00920 (LL671_00920) 208758..209327 + 570 WP_005687747.1 PTS glucitol/sorbitol transporter subunit IIC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10485.91 Da        Isoelectric Point: 9.6577

>T222963 WP_005687756.1 NZ_CP086326:c204652-204392 [Lacticaseibacillus rhamnosus]
MIKTWTDDAWADYMYWHDQNDKRTIKRINQLIQAIDRDPYKGIGKPEPLRYALTGKWSRRIDQENRIIYSIEKNHINIFA
CRTHYS

Download         Length: 261 bp

>T222963 NZ_CP118223:1951100-1951453 [Klebsiella grimontii]
GTGTGGATGATTAAAACAACGGATACGTTTGAGCGCTGGTTTACCTCGCTCAACGATACCGATAGGGCCAGGGTGCTTGC
AGCGTTGTTGGTCCTGCGAGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGCTGAGAGGCTCCCGCTACAGCG
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGAGCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACCGGCATT
GTGCTTTGTGCCGGTAATAAGGTTGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGAGCGGGAATTCAC
GAACTGGCTGAAGACATTCAAAGAGAAGGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10181.39 Da        Isoelectric Point: 4.6755

>AT222963 WP_005687754.1 NZ_CP086326:c204914-204645 [Lacticaseibacillus rhamnosus]
MEATNYSDFRRNLKHYMSQVNEDAEPLLVTAKDDDDNVVVMSKHDFDAIEETLYLLSNPKLMAKIKRGDAQIAAGKAKQH
ELLTDFDHD

Download         Length: 270 bp

>AT222963 NZ_CP118223:1951458-1951760 [Klebsiella grimontii]
ATGGGCAGAACGTTGGAACAGCTGATGGCGGATGAAAAACCGGAAGTCGTGGCTGACGCCCGGGCTATGGCGGCGGACAT
CCTGCTCAACATTCACCTTGCAGAACTGCGTGAGAAAGTACAGAAAACGCAGGTCGAGATGGCGCAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGCATGGAAAAACCAGGCCGTGACCTGAAGCTTTCCACGCTTAAACGCTACGTTGAAGCCACC
GGCGGCAAGCTACGTCTTGATGTTGAGTTGCCGGATGGTTCTCACTACGGATTTGAACTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249N526

Antitoxin

Source ID Structure
AlphaFold DB A0A249N594

References