Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 367759..368425 Replicon chromosome
Accession NZ_CP086287
Organism Citrobacter freundii strain DY2010

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag LMH92_RS01710 Protein ID WP_003847996.1
Coordinates 368108..368425 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag LMH92_RS01705 Protein ID WP_003837894.1
Coordinates 367759..368055 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH92_RS01690 (364935) 364935..365408 - 474 WP_003023524.1 transcription elongation factor GreB -
LMH92_RS01695 (365634) 365634..366353 + 720 WP_001157751.1 two-component system response regulator OmpR -
LMH92_RS01700 (366350) 366350..367702 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
LMH92_RS01705 (367759) 367759..368055 - 297 WP_003837894.1 NadS family protein Antitoxin
LMH92_RS01710 (368108) 368108..368425 - 318 WP_003847996.1 hypothetical protein Toxin
LMH92_RS01715 (368548) 368548..370170 - 1623 WP_044701411.1 phosphoenolpyruvate carboxykinase (ATP) -
LMH92_RS01720 (370549) 370549..372267 + 1719 WP_047715944.1 DUF4153 domain-containing protein -
LMH92_RS01725 (372377) 372377..373255 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T222903 WP_003847996.1 NZ_CP086287:c368425-368108 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T222903 NZ_CP118153:581994-582176 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAATA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATTCCCCACCCGGATGCGGATCTGCCCAAGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT222903 WP_003837894.1 NZ_CP086287:c368055-367759 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT222903 NZ_CP118153:582206-582607 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGCGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCAGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAACCCCGATTACGCCGGTGGCGTGTGGGGCATGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAACTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGTTACCGCTCGCGTTCGGGCTTTCTCGCGGCTGCCGCTTTACGCGAACTCTCCGCCT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References