Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3739392..3739989 Replicon chromosome
Accession NZ_CP086285
Organism Klebsiella pneumoniae strain Kp1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LL004_RS18445 Protein ID WP_004142563.1
Coordinates 3739672..3739989 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LL004_RS18440 Protein ID WP_004142561.1
Coordinates 3739392..3739679 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL004_RS18410 3735599..3735847 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LL004_RS18415 3735865..3736206 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LL004_RS18420 3736237..3737352 - 1116 WP_074422059.1 MBL fold metallo-hydrolase -
LL004_RS18425 3737532..3738113 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LL004_RS18430 3738113..3738481 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LL004_RS18435 3738601..3739254 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LL004_RS18440 3739392..3739679 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LL004_RS18445 3739672..3739989 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LL004_RS18450 3740174..3741217 - 1044 WP_102008192.1 DUF2157 domain-containing protein -
LL004_RS18455 3741887..3742753 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LL004_RS18460 3742862..3744289 + 1428 WP_065800932.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T222895 WP_004142563.1 NZ_CP086285:c3739989-3739672 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T222895 NZ_CP118151:c5709388-5709089 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCCCCGGTGGGCGAAGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATCCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT222895 WP_004142561.1 NZ_CP086285:c3739679-3739392 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT222895 NZ_CP118151:c5709092-5708799 [Pseudomonas chlororaphis]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAAGCCATCGCCCTGTTCATGGCGGACGC
CTTCGAAACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGTGTGGTGGCGCGGGCCAAGGGCATGACTGAGCTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTATCGCTCATTCAGCGAAAAAGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGACTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References