Detailed information of TA system TA222876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5066302..5066904
• Replicon: chromosome
• Accession: NZ_CP086259
• Organism: Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LMH79_RS24680
• Protein ID: WP_000897305.1
• Coordinates: 5066593..5066904 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LMH79_RS24675
• Protein ID: WP_000356397.1
• Coordinates: 5066302..5066592 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH79_RS24650 (5062246)	5062246..5063148	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LMH79_RS24655 (5063145)	5063145..5063780	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LMH79_RS24660 (5063777)	5063777..5064706	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LMH79_RS24665 (5065036)	5065036..5065278	-	243	WP_001087409.1	protein YiiF	-
LMH79_RS24670 (5065498)	5065498..5065716	-	219	WP_001315930.1	CopG family transcriptional regulator	-
LMH79_RS24675 (5066302)	5066302..5066592	-	291	WP_000356397.1	NadS family protein	Antitoxin
LMH79_RS24680 (5066593)	5066593..5066904	-	312	WP_000897305.1	hypothetical protein	Toxin
LMH79_RS24685 (5067133)	5067133..5068041	+	909	WP_001387201.1	alpha/beta hydrolase	-
LMH79_RS24690 (5068105)	5068105..5069046	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LMH79_RS24695 (5069091)	5069091..5069528	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LMH79_RS24700 (5069525)	5069525..5070397	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LMH79_RS24705 (5070391)	5070391..5070990	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T222876 WP_000897305.1 NZ_CP086259:c5066904-5066593 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T222876 NZ_CP118149:c546933-546505 [Pseudomonas chlororaphis]
ATGATAGTACTCGACACAAATGTGTTGTCAGAACTGATGCGCCCTCAACCGGATACGGCGGTATTGCTGTGGATCGAAGC
GCAACCCGTCGAAGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTCCACGGTATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCGCTGGCTATGTTCGAGGAAGACTTCATCGACCGCATCGTGCCCTTCGGAACC
CAAGCGGCTCTTTACTACACCCATCTGGTCTCGCATCGTGAAAGATCAGGCCAGCCGATCAATCTGGTGGATGCGCAAAT
TGCAGCGATATGCCGGGTCCACGACGCCGCGATCGCCACTCGTAACACCAAGGATTTCACCGACACCGGACTGATCGTTA
TCAACCCCTGGCTGCCGCTCGAGGTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT222876 WP_000356397.1 NZ_CP086259:c5066592-5066302 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT222876 NZ_CP118149:c547175-546930 [Pseudomonas chlororaphis]
ATGGCCAGCCTTACGATCCGTAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCATTGAGCAGGACAGCTGACAGCCAAGGCCTGGGCAGCCGTATCA
GGGCACGCTTTGCCGAGACCGGCGGAGTAGAGCTGGAGCTGCCTGAACGTACCGAACCGGCACGCTTGGTGGACCTGGGC
GAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7