Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4097068..4097609 Replicon chromosome
Accession NZ_CP086259
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag LMH79_RS20135 Protein ID WP_000615974.1
Coordinates 4097331..4097609 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LMH79_RS20130 Protein ID WP_000729704.1
Coordinates 4097068..4097328 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS20105 (4092851) 4092851..4093636 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
LMH79_RS20110 (4093608) 4093608..4095320 + 1713 Protein_3943 flagellar biosynthesis protein FlhA -
LMH79_RS20115 (4095425) 4095425..4095703 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
LMH79_RS20120 (4095696) 4095696..4096052 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LMH79_RS20125 (4096109) 4096109..4096882 - 774 WP_000543895.1 C40 family peptidase -
LMH79_RS20130 (4097068) 4097068..4097328 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LMH79_RS20135 (4097331) 4097331..4097609 + 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LMH79_RS20140 (4097765) 4097765..4098505 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LMH79_RS20145 (4098476) 4098476..4099243 - 768 WP_000333380.1 class II glutamine amidotransferase -
LMH79_RS20150 (4099449) 4099449..4100027 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T222871 WP_000615974.1 NZ_CP086259:4097331-4097609 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T222871 NZ_CP118148:c6360413-6360120 [Pseudomonas chlororaphis]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGTCCCTGCGTGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCGTTCAGTGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGCGCCCTGGGGCTGGATATGACGGCGCGGGTGCATGGGGCGTCTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT222871 WP_000729704.1 NZ_CP086259:4097068-4097328 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT222871 NZ_CP118148:c6360709-6360410 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAATGGCCTTGCCGGTGACGTGCCACCGGTGGGCGAGGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTATCGGGTTTACTTCCAGCAGCTTGGTTCGCAACTCGTCATCCTGTTATGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References