Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 679443..680242 Replicon chromosome
Accession NZ_CP086259
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag LMH79_RS03330 Protein ID WP_000347267.1
Coordinates 679443..679907 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LMH79_RS03335 Protein ID WP_001307405.1
Coordinates 679907..680242 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS03300 (674444) 674444..674878 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LMH79_RS03305 (674896) 674896..675774 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LMH79_RS03310 (675764) 675764..676543 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LMH79_RS03315 (676554) 676554..677027 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LMH79_RS03320 (677050) 677050..678330 - 1281 WP_000681935.1 tagatose-bisphosphate aldolase subunit KbaZ -
LMH79_RS03325 (678579) 678579..679388 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LMH79_RS03330 (679443) 679443..679907 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LMH79_RS03335 (679907) 679907..680242 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LMH79_RS03340 (680391) 680391..681962 - 1572 WP_001273741.1 galactarate dehydratase -
LMH79_RS03345 (682336) 682336..683670 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LMH79_RS03350 (683686) 683686..684456 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T222852 WP_000347267.1 NZ_CP086259:c679907-679443 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T222852 NZ_CP118146:c2552053-2551760 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACGCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGCCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTGCGCGGCCACACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT222852 WP_001307405.1 NZ_CP086259:c680242-679907 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT222852 NZ_CP118146:c2551758-2551468 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGTTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCTCGCGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References