Detailed information of TA system TA222843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2273863..2274403
• Replicon: chromosome
• Accession: NZ_CP086254
• Organism: Brachybacterium halotolerans subsp. kimchii strain CBA3105

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: Bra3105_RS10230
• Protein ID: WP_200503265.1
• Coordinates: 2273863..2274123 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: Bra3105_RS10235
• Protein ID: WP_200503266.1
• Coordinates: 2274125..2274403 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra3105_RS10200 (Bra3105_10215)	2269040..2270002	-	963	WP_228356600.1	EamA family transporter	-
Bra3105_RS10205 (Bra3105_10220)	2270074..2271066	+	993	WP_228356601.1	LysR family transcriptional regulator	-
Bra3105_RS10210 (Bra3105_10225)	2271063..2272106	+	1044	WP_228356602.1	cation diffusion facilitator family transporter	-
Bra3105_RS10215 (Bra3105_10230)	2272202..2272621	+	420	WP_200503262.1	YccF domain-containing protein	-
Bra3105_RS10220 (Bra3105_10235)	2272649..2273398	-	750	WP_228356603.1	VOC family protein	-
Bra3105_RS10225 (Bra3105_10240)	2273409..2273807	-	399	WP_228356604.1	serine/threonine protein phosphatase	-
Bra3105_RS10230 (Bra3105_10245)	2273863..2274123	-	261	WP_200503265.1	Txe/YoeB family addiction module toxin	Toxin
Bra3105_RS10235 (Bra3105_10250)	2274125..2274403	-	279	WP_200503266.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
Bra3105_RS10240 (Bra3105_10255)	2274551..2275435	+	885	WP_228356605.1	oxidoreductase	-
Bra3105_RS10245 (Bra3105_10260)	2275501..2276352	+	852	WP_228356606.1	alpha/beta fold hydrolase	-
Bra3105_RS10250 (Bra3105_10265)	2276349..2277662	+	1314	WP_228356607.1	MFS transporter	-
Bra3105_RS10255 (Bra3105_10275)	2277689..2278129	+	441	WP_228356608.1	VOC family protein	-
Bra3105_RS10260 (Bra3105_10280)	2278162..2278740	-	579	WP_228356609.1	TetR/AcrR family transcriptional regulator	-
Bra3105_RS10265 (Bra3105_10285)	2278884..2279234	+	351	WP_228356610.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10315.70 Da
• Isoelectric Point: 8.8893

>T222843 WP_200503265.1 NZ_CP086254:c2274123-2273863 [Brachybacterium halotolerans subsp. kimchii]
MRLVWDENAWEDYLWWQAQDRRVLRRINALLKDIARNGNEGIGKPEALKHDFAGYWSRRITDEHRLVYKLVGADEVRIAA
CRYHYG

Nucleotide

• Length: 261 bp

>T222843 NZ_CP118145:c2535755-2535573 [Pseudomonas chlororaphis]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTCACCGGCAGCCATCACCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCGCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCA
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10423.54 Da
• Isoelectric Point: 4.3325

>AT222843 WP_200503266.1 NZ_CP086254:c2274403-2274125 [Brachybacterium halotolerans subsp. kimchii]
MKTMSYTESRARYAEVLDGVVDDREEVVVTRAGHDPVVILSLEDYESLRETAYLMRSPRNARRLLDAMERLESGAGSEHE
LIEDNGPDPDRA

Nucleotide

• Length: 279 bp

>AT222843 NZ_CP118145:c2535537-2535136 [Pseudomonas chlororaphis]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACGGCTACCGGCATCCAGATTCCCGACATTCCTGGCGCGGT
CACCGCCGGCGATACCTTCGAGGCCGCGTATAGCGCCGCCATCGAAATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
AGGGACAAGCCATTCCCCTGCCCTCGCCCACCGGCATCCACCGCGCCAACCCCGACTTCGAAGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGTTACGTGATTCAACGCAT
CGACCGCTTTGTGCGCGAGCACAACATCAAGAGCCGGTCGTCTTTCCTGGCGGATGCCGCGATGGAAAAGCTGGGGCGTT
AA