Detailed information of TA system TA222823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 28547..29219
• Replicon: chromosome
• Accession: NZ_CP086253
• Organism: Pectobacterium aquaticum strain A212-S19-A16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DMB82_RS00135
• Protein ID: WP_116164509.1
• Coordinates: 28833..29219 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: DMB82_RS00130
• Protein ID: WP_010281167.1
• Coordinates: 28547..28846 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB82_RS00110 (DMB82_0000110)	23800..24789	+	990	WP_102117952.1	nucleoside hydrolase	-
DMB82_RS00115 (DMB82_0000115)	24789..25766	+	978	WP_102117951.1	ABC transporter permease	-
DMB82_RS00120 (DMB82_0000120)	25763..26671	+	909	WP_010296898.1	ABC transporter permease	-
DMB82_RS00125 (DMB82_0000125)	26711..28510	+	1800	WP_102117950.1	ABC transporter ATP-binding protein	-
DMB82_RS00130 (DMB82_0000130)	28547..28846	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
DMB82_RS00135 (DMB82_0000135)	28833..29219	-	387	WP_116164509.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DMB82_RS00140 (DMB82_0000140)	29541..30239	+	699	WP_039485183.1	D-galactonate utilization transcriptional regulator DgoR	-
DMB82_RS00145 (DMB82_0000145)	30236..31132	+	897	WP_116164507.1	2-dehydro-3-deoxygalactonokinase	-
DMB82_RS00150 (DMB82_0000150)	31116..31733	+	618	WP_116164505.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
DMB82_RS00155 (DMB82_0000155)	31730..32878	+	1149	WP_010281150.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14814.80 Da
• Isoelectric Point: 4.6348

>T222823 WP_116164509.1 NZ_CP086253:c29219-28833 [Pectobacterium aquaticum]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEILAHLSILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVSGNPFRTFFAFD
RTRKAIVLCAGDKKGEDEKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T222823 NZ_CP118143:c1778124-1777942 [Pseudomonas chlororaphis]
GTGGACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGGGGCAGTCACCACCATTT
CAAGCATCCCCATAAACCGGGGCTGGTGACCGTTCCCCATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGTATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT222823 WP_010281167.1 NZ_CP086253:c28846-28547 [Pectobacterium aquaticum]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT222823 NZ_CP118143:c1777909-1777502 [Pseudomonas chlororaphis]
ATGCTTTACCCGATTGCAATTTCAATGGGCGATGACGAACACGCCTGGGGGGTGGAAGTCCCCGATATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCTGAAG
ACGGTGCCCCCATCCCGCACGCCAACAAGGTCACCCTGCACGCGGCCAACCCCCGGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACTTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCCAAGAGCCGCTCGGGTTTTCTCGCTTCGGCTGCACTGAAGGTATTGCAGC
AAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A433N287