Detailed information of TA system TA222815

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86855..87281
• Replicon: plasmid p779-2
• Accession: NZ_CP086222
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LMB17_RS26590
• Protein ID: WP_001372321.1
• Coordinates: 86855..86980 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	87057..87281 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS26550 (82224)	82224..82913	-	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-
LMB17_RS26555 (83100)	83100..83483	-	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LMB17_RS26560 (83804)	83804..84406	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
LMB17_RS26565 (84703)	84703..85524	-	822	WP_001234469.1	DUF932 domain-containing protein	-
LMB17_RS26570 (85646)	85646..85933	-	288	WP_000107535.1	hypothetical protein	-
LMB17_RS26575 (85958)	85958..86164	-	207	WP_000547939.1	hypothetical protein	-
LMB17_RS26580 (86234)	86234..86407	+	174	Protein_84	hypothetical protein	-
LMB17_RS26585 (86405)	86405..86635	-	231	WP_071587244.1	hypothetical protein	-
LMB17_RS26590 (86855)	86855..86980	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LMB17_RS26595 (86922)	86922..87071	-	150	Protein_87	plasmid maintenance protein Mok	-
- (87057)	87057..87281	-	225	NuclAT_0	-	Antitoxin
- (87057)	87057..87281	-	225	NuclAT_0	-	Antitoxin
- (87057)	87057..87281	-	225	NuclAT_0	-	Antitoxin
- (87057)	87057..87281	-	225	NuclAT_0	-	Antitoxin
LMB17_RS26600 (87093)	87093..87281	+	189	WP_001299721.1	hypothetical protein	-
LMB17_RS26605 (87250)	87250..88012	-	763	Protein_89	plasmid SOS inhibition protein A	-
LMB17_RS26610 (88009)	88009..88443	-	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
LMB17_RS26615 (88498)	88498..90456	-	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
LMB17_RS26620 (90522)	90522..90755	-	234	WP_000005990.1	DUF905 family protein	-
LMB17_RS26625 (90818)	90818..91357	-	540	WP_000290791.1	single-stranded DNA-binding protein	-
LMB17_RS26630 (91830)	91830..92078	-	249	WP_071606928.1	hypothetical protein	-
LMB17_RS26635 (92000)	92000..92230	-	231	WP_001333093.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T222815 WP_001372321.1 NZ_CP086222:c86980-86855 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T222815 NZ_CP118142:4957041-4957223 [Pseudomonas chlororaphis]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGTAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAAGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT222815 NZ_CP086222:c87281-87057 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin