222813

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 5008662..5009203 Replicon chromosome
Accession NZ_CP086220
Organism Escherichia coli strain 779

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag LMB17_RS24320 Protein ID WP_000615982.1
Coordinates 5008662..5008940 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LMB17_RS24325 Protein ID WP_000729704.1
Coordinates 5008943..5009203 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMB17_RS24305 (5006244) 5006244..5006822 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
LMB17_RS24310 (5007028) 5007028..5007795 + 768 WP_000333380.1 class II glutamine amidotransferase -
LMB17_RS24315 (5007766) 5007766..5008506 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LMB17_RS24320 (5008662) 5008662..5008940 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LMB17_RS24325 (5008943) 5008943..5009203 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LMB17_RS24330 (5009389) 5009389..5010162 + 774 WP_000543899.1 C40 family peptidase -
LMB17_RS24335 (5010219) 5010219..5010575 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LMB17_RS24340 (5010568) 5010568..5010846 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LMB17_RS24345 (5010951) 5010951..5013044 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
LMB17_RS24350 (5013028) 5013028..5014167 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T222813 WP_000615982.1 NZ_CP086220:c5008940-5008662 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T222813 NZ_CP118142:4942404-4942697 [Pseudomonas chlororaphis]
ATGGAATACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCAGACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATAGGGGCGCCGGCTACCGCATCTATTTCACGGTTCGCGGCAACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT222813 WP_000729704.1 NZ_CP086220:c5009203-5008943 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT222813 NZ_CP118142:4942699-4942989 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGTTGTGCACCACAGTGCCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References