Detailed information of TA system TA222810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4067006..4067608
• Replicon: chromosome
• Accession: NZ_CP086220
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LMB17_RS19825
• Protein ID: WP_000897305.1
• Coordinates: 4067006..4067317 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LMB17_RS19830
• Protein ID: WP_000356397.1
• Coordinates: 4067318..4067608 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS19800 (4062920)	4062920..4063519	+	600	WP_001295269.1	glucose-1-phosphatase	-
LMB17_RS19805 (4063513)	4063513..4064385	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LMB17_RS19810 (4064382)	4064382..4064819	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LMB17_RS19815 (4064864)	4064864..4065805	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LMB17_RS19820 (4065869)	4065869..4066777	-	909	WP_001162704.1	alpha/beta hydrolase	-
LMB17_RS19825 (4067006)	4067006..4067317	+	312	WP_000897305.1	hypothetical protein	Toxin
LMB17_RS19830 (4067318)	4067318..4067608	+	291	WP_000356397.1	NadS family protein	Antitoxin
LMB17_RS19835 (4068194)	4068194..4068412	+	219	WP_001295676.1	CopG family transcriptional regulator	-
LMB17_RS19840 (4068631)	4068631..4068873	+	243	WP_001087409.1	protein YiiF	-
LMB17_RS19845 (4069203)	4069203..4070132	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LMB17_RS19850 (4070129)	4070129..4070764	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LMB17_RS19855 (4070761)	4070761..4071663	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T222810 WP_000897305.1 NZ_CP086220:4067006-4067317 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T222810 NZ_CP118141:c6266251-6265958 [Pseudomonas chlororaphis]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAAGCCATCGCCCTGTTCATGGCGGACGC
CTTCGAAACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGTGTGGTGGCGCGGGCCAAGGGCATGACTGAGCTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTATCGCTCATTCAGCGAAAAAGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGACTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT222810 WP_000356397.1 NZ_CP086220:4067318-4067608 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT222810 NZ_CP118141:c6266547-6266248 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCCCCGGTGGGCGAAGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATCCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGCCGATCATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7