Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3264267..3265066 Replicon chromosome
Accession NZ_CP086220
Organism Escherichia coli strain 779

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag LMB17_RS15905 Protein ID WP_000347266.1
Coordinates 3264602..3265066 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LMB17_RS15900 Protein ID WP_001307405.1
Coordinates 3264267..3264602 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMB17_RS15885 (3260052) 3260052..3260822 - 771 WP_072644821.1 2-dehydro-3-deoxyglucarate aldolase -
LMB17_RS15890 (3260838) 3260838..3262172 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LMB17_RS15895 (3262547) 3262547..3264118 + 1572 WP_001273741.1 galactarate dehydratase -
LMB17_RS15900 (3264267) 3264267..3264602 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LMB17_RS15905 (3264602) 3264602..3265066 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LMB17_RS15910 (3265121) 3265121..3265930 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LMB17_RS15915 (3266179) 3266179..3267459 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
LMB17_RS15920 (3267482) 3267482..3267955 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LMB17_RS15925 (3267966) 3267966..3268745 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LMB17_RS15930 (3268735) 3268735..3269613 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
LMB17_RS15935 (3269631) 3269631..3270065 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3253611..3265066 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T222808 WP_000347266.1 NZ_CP086220:3264602-3265066 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T222808 NZ_CP118141:c2648008-2647718 [Pseudomonas chlororaphis]
ATGTCCGACACACTGCTTGCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT222808 WP_001307405.1 NZ_CP086220:3264267-3264602 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT222808 NZ_CP118141:c2648303-2648010 [Pseudomonas chlororaphis]
ATGAATTACGAGATCCTGCAGACGACCGTCTTTGCCCGATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATATGGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACGCTGATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTACCTGGCCAAGGAGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References