Detailed information of TA system TA222802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1948305..1948807
• Replicon: chromosome
• Accession: NZ_CP086220
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: LMB17_RS09765
• Protein ID: WP_000767829.1
• Coordinates: 1948305..1948559 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LMB17_RS09770
• Protein ID: WP_001259255.1
• Coordinates: 1948556..1948807 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS09735 (1943320)	1943320..1943547	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
LMB17_RS09740 (1943561)	1943561..1944619	-	1059	WP_023568741.1	thiosulfate utilization transporter TsuA/YeeE	-
LMB17_RS09745 (1944798)	1944798..1946156	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
LMB17_RS09750 (1946146)	1946146..1946208	-	63	WP_010723108.1	membrane protein YoeI	-
LMB17_RS09755 (1946423)	1946423..1947352	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
LMB17_RS09760 (1947398)	1947398..1948222	-	825	WP_000754763.1	SDR family oxidoreductase	-
LMB17_RS09765 (1948305)	1948305..1948559	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LMB17_RS09770 (1948556)	1948556..1948807	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LMB17_RS09775 (1949089)	1949089..1949139	+	51	WP_001364200.1	his operon leader peptide	-
LMB17_RS09780 (1949285)	1949285..1950184	+	900	WP_061093169.1	ATP phosphoribosyltransferase	-
LMB17_RS09785 (1950190)	1950190..1951494	+	1305	WP_001515511.1	histidinol dehydrogenase	-
LMB17_RS09790 (1951491)	1951491..1952561	+	1071	WP_000108943.1	histidinol-phosphate transaminase	-
LMB17_RS09795 (1952561)	1952561..1953628	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T222802 WP_000767829.1 NZ_CP086220:c1948559-1948305 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T222802 NZ_CP118140:c4692040-4691759 [Pseudomonas chlororaphis]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAACCCCCATGTCCCGGCCGATCGCCTGAGCGGTTTGGGCAATGCCTACAAAATCA
AACTGCGCACGGCGGGTTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGTTGGTGGTGACGGTCATTGCCGTGGGCAAG
CGAGAGCGGGGGAGTGTCTATCAGCAAGCCAGCAAGCGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT222802 WP_001259255.1 NZ_CP086220:c1948807-1948556 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT222802 NZ_CP118140:c4692278-4692030 [Pseudomonas chlororaphis]
ATGACCCATGTCCTTCTGTCCAACGTCGTTGCGAGCATTTCCGAGCTCAAGAAAAACCCCATGGGCACCGTTGCGGCCGG
TGGCGGTTCCTCCGTGGCCATCCTCAATCGCAACGAACCGGCGTTTTACTGCGTTCCGGCCAAGGAATACGAAGCCATGA
TGGAGCGTCTGGAAGATATGGAGCTGCTCGCCTTGTGCCGTGAACGAGAGAATGATCCGACCATCAAGGTTTCGCTCGAT
GACTTATGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0