Detailed information of TA system TA222787

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1152713..1153084
• Replicon: chromosome
• Accession: NZ_CP086220
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: LMB17_RS05660
• Protein ID: WP_001317028.1
• Coordinates: 1152890..1153084 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1152713..1152891 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS05630 (1148465)	1148465..1148638	+	174	WP_001296046.1	protein YnaL	-
LMB17_RS05635 (1148668)	1148668..1150041	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
LMB17_RS05640 (1150170)	1150170..1151105	-	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LMB17_RS05645 (1151157)	1151157..1152392	-	1236	WP_000040852.1	site-specific integrase	-
LMB17_RS05650 (1152394)	1152394..1152609	-	216	WP_000079604.1	excisionase XisR	-
- (1152713)	1152713..1152891	+	179	NuclAT_0	-	Antitoxin
- (1152713)	1152713..1152891	+	179	NuclAT_0	-	Antitoxin
- (1152713)	1152713..1152891	+	179	NuclAT_0	-	Antitoxin
- (1152713)	1152713..1152891	+	179	NuclAT_0	-	Antitoxin
LMB17_RS05655 (1152688)	1152688..1152897	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
LMB17_RS05660 (1152890)	1152890..1153084	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LMB17_RS05665 (1153141)	1153141..1153950	-	810	WP_000166319.1	recombination protein RecT	-
LMB17_RS05670 (1153943)	1153943..1156543	-	2601	WP_000105127.1	exodeoxyribonuclease VIII	-
LMB17_RS05675 (1156645)	1156645..1156920	-	276	WP_000632297.1	protein RacC	-
LMB17_RS05680 (1156995)	1156995..1157165	-	171	WP_001352098.1	YdaE family protein	-
LMB17_RS05685 (1157165)	1157165..1157386	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1151157..1177530	26373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T222787 WP_001317028.1 NZ_CP086220:c1153084-1152890 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T222787 NZ_CP118138:7038766-7039056 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACAGTGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACAGTGCCCCGTGGCTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT222787 NZ_CP086220:1152713-1152891 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin