Detailed information of TA system TA222772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2452257..2452774
• Replicon: chromosome
• Accession: NZ_CP086215
• Organism: Staphylococcus aureus strain Patient 14 Isolate 02

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: LLZ98_RS12115
• Protein ID: WP_000113265.1
• Coordinates: 2452508..2452774 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: LLZ98_RS12110
• Protein ID: WP_000584499.1
• Coordinates: 2452257..2452508 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLZ98_RS12080 (2447955)	2447955..2448674	+	720	WP_000479576.1	amino acid ABC transporter permease	-
LLZ98_RS12085 (2448671)	2448671..2449402	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
LLZ98_RS12090 (2449499)	2449499..2450749	-	1251	WP_001012221.1	FemA/FemB family glycyltransferase FmhA	-
LLZ98_RS12095 (2451004)	2451004..2451366	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
LLZ98_RS12100 (2451385)	2451385..2451984	+	600	WP_000162810.1	DsbA family protein	-
LLZ98_RS12105 (2452012)	2452012..2452092	+	81	WP_223197974.1	hypothetical protein	-
LLZ98_RS12110 (2452257)	2452257..2452508	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LLZ98_RS12115 (2452508)	2452508..2452774	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
LLZ98_RS12120 (2452809)	2452809..2452880	+	72	Protein_2359	hypothetical protein	-
LLZ98_RS12125 (2453069)	2453069..2454616	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
LLZ98_RS12130 (2454802)	2454802..2455269	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
LLZ98_RS12135 (2455353)	2455353..2455535	+	183	WP_000230292.1	hypothetical protein	-
LLZ98_RS12140 (2455752)	2455752..2456576	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
LLZ98_RS12145 (2456810)	2456810..2457346	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T222772 WP_000113265.1 NZ_CP086215:2452508-2452774 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T222772 NZ_CP118136:6592586-6592768 [Pseudomonas chlororaphis]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGCGGAGTTCCAACCCGGCAAGGGCAGTCACTTCAAGGT
CACCTTGAATGGCAAGTCAACCGTGTTTCCAGACCACGGAGCCAAGGAAATGGGCGAAGGGTTAAGGAAGTCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT222772 WP_000584499.1 NZ_CP086215:2452257-2452508 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT222772 NZ_CP118136:6592801-6593226 [Pseudomonas chlororaphis]
ATGTTCAAGTACGCATTGGAAATTCACGAAGAGCCCGGCACCGTCTGGCTTTCGTGTGAGGAGATTCCAGAATTTCACGC
TGCCGGCGACACCGTAGGCGAGGCGCTGGATAGTGTTTTGGATGCGCTCGAAACGGCTCTGTCTATCTATGTCGATGAAC
GCCGCCCGATTCCATCTGGTAACGCCGAGGCTCAGGCAGGGCACGTTCTGTTATGCCTTCCAGCCCTCACCGCGGCGAAG
GTTGCACTGTGGAACGCGGTGTTGGATGAAGGCACCACCAAGGCAGAGCTGGCGCGCCGCCTGGGGATTCAGCGGCCGCA
GGTGGACCGCTTGCTGGACTTCCTGCACGACTCGAAAATCGAAAACGTCGAGCGCGCCCTGCAGGAACTCGGTCGGCGGA
TCTCTATCACCGTCGAAGCTGCATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60