Detailed information of TA system TA222758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6154826..6155421
• Replicon: chromosome
• Accession: NZ_CP086213
• Organism: Pseudomonas aeruginosa strain Pa3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: LLJ53_RS29290
• Protein ID: WP_003117425.1
• Coordinates: 6155143..6155421 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LLJ53_RS29285
• Protein ID: WP_003099268.1
• Coordinates: 6154826..6155131 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLJ53_RS29250 (LLJ53_29245)	6150049..6150300	-	252	WP_003115979.1	hypothetical protein	-
LLJ53_RS29255 (LLJ53_29250)	6150422..6150856	-	435	WP_023087663.1	hypothetical protein	-
LLJ53_RS29260 (LLJ53_29255)	6151372..6151662	-	291	WP_033896069.1	DUF5447 family protein	-
LLJ53_RS29265 (LLJ53_29260)	6151838..6152110	-	273	WP_004352675.1	hypothetical protein	-
LLJ53_RS29270 (LLJ53_29265)	6152557..6152892	+	336	WP_025297674.1	hypothetical protein	-
LLJ53_RS29275 (LLJ53_29270)	6152945..6154036	+	1092	WP_124135598.1	hypothetical protein	-
LLJ53_RS29280 (LLJ53_29275)	6154097..6154489	-	393	WP_025297673.1	hypothetical protein	-
LLJ53_RS29285 (LLJ53_29280)	6154826..6155131	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
LLJ53_RS29290 (LLJ53_29285)	6155143..6155421	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LLJ53_RS29295 (LLJ53_29290)	6155746..6157974	+	2229	WP_003113525.1	TonB-dependent receptor	-
LLJ53_RS29300 (LLJ53_29295)	6158044..6158691	-	648	WP_003095021.1	carbonate dehydratase	-
LLJ53_RS29305 (LLJ53_29300)	6158753..6159991	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T222758 WP_003117425.1 NZ_CP086213:c6155421-6155143 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T222758 NZ_CP118135:c2091153-2090872 [Pseudomonas chlororaphis]
ATGACTTATAAACTCCAGTTTCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAAGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGCCCGCGGATGCCCTTCACGGCATGGCGGACTGCTACAAGATCA
AGCTCAAGGCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTGGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGCGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT222758 WP_003099268.1 NZ_CP086213:c6155131-6154826 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT222758 NZ_CP118135:c2091388-2091143 [Pseudomonas chlororaphis]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAACCCCTCGGCTGTCCTGAGCGGCGCACA
CGGTGGTCCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGGCACGAGACCCCAGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5