Detailed information of TA system TA222693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2540020..2540609
• Replicon: chromosome
• Accession: NZ_CP086193
• Organism: Xanthomonas oryzae strain GDV

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: LL918_RS11905
• Protein ID: WP_012443754.1
• Coordinates: 2540328..2540609 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL918_RS11900
• Protein ID: WP_011260746.1
• Coordinates: 2540020..2540310 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL918_RS11870 (LL918_11855)	2535173..2535781	-	609	WP_011409746.1	DUF4123 domain-containing protein	-
LL918_RS11875 (LL918_11860)	2535784..2536197	-	414	WP_228383576.1	PAAR domain-containing protein	-
LL918_RS11880 (LL918_11865)	2536334..2536660	-	327	WP_228329596.1	hypothetical protein	-
LL918_RS11885 (LL918_11870)	2536915..2537247	+	333	WP_011260743.1	hypothetical protein	-
LL918_RS11890 (LL918_11875)	2537407..2537594	+	188	Protein_2273	hypothetical protein	-
LL918_RS11895 (LL918_11880)	2537697..2539091	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
LL918_RS11900 (LL918_11885)	2540020..2540310	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
LL918_RS11905 (LL918_11890)	2540328..2540609	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL918_RS11910 (LL918_11895)	2540704..2542956	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T222693 WP_012443754.1 NZ_CP086193:c2540609-2540328 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T222693 NZ_CP118021:c1477443-1477093 [Bacillus spizizenii]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT222693 WP_011260746.1 NZ_CP086193:c2540310-2540020 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT222693 NZ_CP118021:c1477729-1477448 [Bacillus spizizenii]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5