Detailed information of TA system TA222687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1480414..1481003
• Replicon: chromosome
• Accession: NZ_CP086191
• Organism: Xanthomonas oryzae strain JS49-6

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: LL928_RS07020
• Protein ID: WP_012443754.1
• Coordinates: 1480722..1481003 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL928_RS07015
• Protein ID: WP_011260746.1
• Coordinates: 1480414..1480704 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL928_RS06985 (LL928_06980)	1475558..1476175	-	618	WP_082325600.1	DUF4123 domain-containing protein	-
LL928_RS06990 (LL928_06985)	1476178..1476600	-	423	WP_042465811.1	PAAR domain-containing protein	-
LL928_RS06995 (LL928_06990)	1476728..1477054	-	327	WP_228329596.1	hypothetical protein	-
LL928_RS07000 (LL928_06995)	1477309..1477641	+	333	WP_011260743.1	hypothetical protein	-
LL928_RS07005 (LL928_07000)	1477801..1477988	+	188	Protein_1335	hypothetical protein	-
LL928_RS07010 (LL928_07005)	1478091..1479485	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
LL928_RS07015 (LL928_07010)	1480414..1480704	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
LL928_RS07020 (LL928_07015)	1480722..1481003	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL928_RS07025 (LL928_07020)	1481098..1483350	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T222687 WP_012443754.1 NZ_CP086191:c1481003-1480722 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T222687 NZ_CP118014:4527056-4527334 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT222687 WP_011260746.1 NZ_CP086191:c1480704-1480414 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT222687 NZ_CP118014:4526793-4527053 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5