Detailed information of TA system TA222679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1952232..1952821
• Replicon: chromosome
• Accession: NZ_CP086185
• Organism: Xanthomonas oryzae strain PXO112

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: LL920_RS09080
• Protein ID: WP_012443754.1
• Coordinates: 1952540..1952821 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL920_RS09075
• Protein ID: WP_011260746.1
• Coordinates: 1952232..1952522 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL920_RS09045 (LL920_09025)	1947516..1947992	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
LL920_RS09050 (LL920_09030)	1947995..1948417	-	423	WP_033013301.1	PAAR domain-containing protein	-
LL920_RS09055 (LL920_09035)	1948567..1948872	-	306	WP_224229802.1	hypothetical protein	-
LL920_RS09060 (LL920_09040)	1949127..1949459	+	333	WP_011260743.1	hypothetical protein	-
LL920_RS09065 (LL920_09045)	1949619..1949806	+	188	Protein_1735	hypothetical protein	-
LL920_RS09070 (LL920_09050)	1949909..1951303	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
LL920_RS09075 (LL920_09055)	1952232..1952522	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
LL920_RS09080 (LL920_09060)	1952540..1952821	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL920_RS09085 (LL920_09065)	1952916..1955168	-	2253	WP_012443753.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T222679 WP_012443754.1 NZ_CP086185:c1952821-1952540 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T222679 NZ_CP118014:2962539-2962826 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT222679 WP_011260746.1 NZ_CP086185:c1952522-1952232 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT222679 NZ_CP118014:2962300-2962539 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5