Detailed information of TA system TA222668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 464060..464649
• Replicon: chromosome
• Accession: NZ_CP086183
• Organism: Xanthomonas oryzae strain PXO341

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: LL922_RS02150
• Protein ID: WP_012443754.1
• Coordinates: 464060..464341 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL922_RS02155
• Protein ID: WP_011260746.1
• Coordinates: 464359..464649 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL922_RS02145 (LL922_02145)	461713..463965	+	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-
LL922_RS02150 (LL922_02150)	464060..464341	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL922_RS02155 (LL922_02155)	464359..464649	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
LL922_RS02160 (LL922_02160)	465578..466972	+	1395	WP_027703652.1	type III secretion system effector XopQ	-
LL922_RS02165 (LL922_02165)	467075..467262	-	188	Protein_422	hypothetical protein	-
LL922_RS02170 (LL922_02170)	467422..467754	-	333	WP_011260743.1	hypothetical protein	-
LL922_RS02175 (LL922_02175)	468009..468335	+	327	WP_228329596.1	hypothetical protein	-
LL922_RS02180 (LL922_02180)	468463..468885	+	423	WP_042465811.1	PAAR domain-containing protein	-
LL922_RS02185 (LL922_02185)	468888..469496	+	609	WP_011409746.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T222668 WP_012443754.1 NZ_CP086183:464060-464341 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T222668 NZ_CP118014:c386669-386358 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT222668 WP_011260746.1 NZ_CP086183:464359-464649 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT222668 NZ_CP118014:c386357-386067 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5