Detailed information of TA system TA222667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4853662..4854251
• Replicon: chromosome
• Accession: NZ_CP086182
• Organism: Xanthomonas oryzae strain PXO347

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: LL921_RS22875
• Protein ID: WP_012443754.1
• Coordinates: 4853662..4853943 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL921_RS22880
• Protein ID: WP_011260746.1
• Coordinates: 4853961..4854251 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL921_RS22870 (LL921_22825)	4851315..4853567	+	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-
LL921_RS22875 (LL921_22830)	4853662..4853943	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL921_RS22880 (LL921_22835)	4853961..4854251	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
LL921_RS22885 (LL921_22840)	4855180..4856574	+	1395	WP_027703652.1	type III secretion system effector XopQ	-
LL921_RS22890 (LL921_22845)	4856677..4856864	-	188	Protein_4376	hypothetical protein	-
LL921_RS22895 (LL921_22850)	4857024..4857356	-	333	WP_011260743.1	hypothetical protein	-
LL921_RS22900 (LL921_22855)	4857611..4857937	+	327	WP_228329596.1	hypothetical protein	-
LL921_RS22905 (LL921_22860)	4858065..4858487	+	423	WP_042465811.1	PAAR domain-containing protein	-
LL921_RS22910 (LL921_22865)	4858490..4859107	+	618	WP_082325600.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T222667 WP_012443754.1 NZ_CP086182:4853662-4853943 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T222667 NZ_CP118014:c73401-73228 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT222667 WP_011260746.1 NZ_CP086182:4853961-4854251 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT222667 NZ_CP118014:c73725-73429 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5