Detailed information of TA system TA222662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4671028..4671617
• Replicon: chromosome
• Accession: NZ_CP086181
• Organism: Xanthomonas oryzae strain PXO349

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: LL917_RS21935
• Protein ID: WP_012443754.1
• Coordinates: 4671336..4671617 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL917_RS21930
• Protein ID: WP_011260746.1
• Coordinates: 4671028..4671318 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL917_RS21900 (LL917_21890)	4666172..4666789	-	618	WP_082325600.1	DUF4123 domain-containing protein	-
LL917_RS21905 (LL917_21895)	4666792..4667214	-	423	WP_042465811.1	PAAR domain-containing protein	-
LL917_RS21910 (LL917_21900)	4667342..4667668	-	327	WP_228329596.1	hypothetical protein	-
LL917_RS21915 (LL917_21905)	4667923..4668255	+	333	WP_011260743.1	hypothetical protein	-
LL917_RS21920 (LL917_21910)	4668415..4668602	+	188	Protein_4183	hypothetical protein	-
LL917_RS21925 (LL917_21915)	4668705..4670099	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
LL917_RS21930 (LL917_21920)	4671028..4671318	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
LL917_RS21935 (LL917_21925)	4671336..4671617	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL917_RS21940 (LL917_21930)	4671712..4673964	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T222662 WP_012443754.1 NZ_CP086181:c4671617-4671336 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T222662 NZ_CP117993:4580453-4580824 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGAGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT222662 WP_011260746.1 NZ_CP086181:c4671318-4671028 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT222662 NZ_CP117993:4580208-4580450 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5