Detailed information of TA system TA222655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2377587..2378176
• Replicon: chromosome
• Accession: NZ_CP086180
• Organism: Xanthomonas oryzae strain PXO363

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: LL927_RS11315
• Protein ID: WP_012443754.1
• Coordinates: 2377587..2377868 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LL927_RS11320
• Protein ID: WP_011260746.1
• Coordinates: 2377886..2378176 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL927_RS11310 (LL927_11285)	2375240..2377492	+	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-
LL927_RS11315 (LL927_11290)	2377587..2377868	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL927_RS11320 (LL927_11295)	2377886..2378176	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
LL927_RS11325 (LL927_11300)	2379105..2380499	+	1395	WP_027703652.1	type III secretion system effector XopQ	-
LL927_RS11330 (LL927_11305)	2380602..2380789	-	188	Protein_2168	hypothetical protein	-
LL927_RS11335 (LL927_11310)	2380949..2381281	-	333	WP_011260743.1	hypothetical protein	-
LL927_RS11340 (LL927_11315)	2381536..2381862	+	327	WP_228329596.1	hypothetical protein	-
LL927_RS11345 (LL927_11320)	2381999..2382412	+	414	WP_228383576.1	PAAR domain-containing protein	-
LL927_RS11350 (LL927_11325)	2382415..2383032	+	618	WP_082325600.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T222655 WP_012443754.1 NZ_CP086180:2377587-2377868 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T222655 NZ_CP117993:717222-717632 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTACGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT222655 WP_011260746.1 NZ_CP086180:2377886-2378176 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT222655 NZ_CP117993:716975-717241 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5